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Application of long-chain non-coding RNA LINC00559 serving as biomarker in preparation of lung adenocarcinoma prognosis detection preparation

A long-chain non-coding and biomarker technology, applied in the field of preparation of lung adenocarcinoma prognosis detection reagents, can solve the problem of inability to adapt to the prognosis of lung adenocarcinoma patients, no specific indicators, and the prognosis of lung adenocarcinoma patients There are no reference standards and other issues, achieving far-reaching clinical significance and promotional effects

Active Publication Date: 2018-06-22
XIANGYA HOSPITAL CENT SOUTH UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, there is no reference standard for the prognosis of patients with lung adenocarcinoma, and there is no specific index, which is far from meeting the needs of the prognosis of patients with lung adenocarcinoma

Method used

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  • Application of long-chain non-coding RNA LINC00559 serving as biomarker in preparation of lung adenocarcinoma prognosis detection preparation
  • Application of long-chain non-coding RNA LINC00559 serving as biomarker in preparation of lung adenocarcinoma prognosis detection preparation
  • Application of long-chain non-coding RNA LINC00559 serving as biomarker in preparation of lung adenocarcinoma prognosis detection preparation

Examples

Experimental program
Comparison scheme
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Embodiment 1

[0013] Example 1 Preparation of long-chain non-coding RNA LINC00559 for lung adenocarcinoma prognosis detection kit (50 reactions)

[0014] 1. RNA stabilization solution 50ml

[0015] 2. Isopropanol 100ml

[0016] 3. Chloroform 100ml

[0017] 4. Trizol 50ml

[0018] 5. Enzyme-free water 10ml

[0019] 6. 1μM random reverse transcription primer 50ul

[0020] 7. 5× reverse transcription buffer 200ml

[0021] 8.10mM base triphosphate deoxynucleotide 100ul

[0022] 9. 40U / μl RNase inhibitor 500ul

[0023] 10. 200U / μl MMLV reverse transcriptase 50ul

[0024] 11. Premix Ex Taq 50ul

[0025] 12. 10 μM long non-coding RNA LINC00559 real-time fluorescent quantitative PCR specific primers:

[0026] Forward primer 5'-CAATGGACTACCATTTATGACTAC-3' (SEQ ID NO.2),

[0027] Reverse primer 5'-CGGATCTACTTCTCCAGCAGATTTG-3' (SEQ ID NO.3),

[0028] 13. 10μM internal reference GAPDH specific primers for real-time fluorescence quantitative PCR:

[0029] The forward primer is 5'-CATCCATGACA...

Embodiment 2

[0031] Example 2 Detection of long non-coding RNA LINC00559 in lung adenocarcinoma tissue

[0032] 1. Preservation of lung adenocarcinoma tissue: collect the lung adenocarcinoma tissue to be tested and store it in a cryopreservation tube filled with RNA stabilization solution, and put it in a -80°C refrigerator for later use.

[0033] 2. Extraction of RNA in tissues: Take an appropriate amount of specimen, add liquid nitrogen to the mortar after baking at 180°C for 6-8 hours, grind the specimen, grind it to powder, add 1ml Trizol mortar specimen in the mortar, and grind it into After liquid, transfer to a centrifuge tube, add 200μl / ml chloroform to the centrifuge tube, shake by hand for 15-30s, place on ice for 5min, centrifuge at 12000g at 4°C for 15min; carefully take the upper aqueous phase into a new centrifuge tube, add Mix with 0.5ml of pre-cooled isopropanol, put it in a refrigerator at -20°C for 20min, centrifuge at 12000g at 4°C for 10min; discard the supernatant, add...

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Abstract

The invention discloses application of long-chain non-coding RNA LINC00559 serving as a biomarker in preparation of a lung adenocarcinoma prognosis detection preparation. The expression level of the long-chain non-coding RNA LINC00559 in a patient suffering from lung adenocarcinoma can be detected, a strong molecular biology basic is provided for prognosis prediction of the patient suffering fromlung adenocarcinoma, and profound and lasting clinical significance and good generalization performance are achieved.

Description

technical field [0001] The invention belongs to the field of tumor molecular biology, and specifically relates to the application of long-chain non-coding RNA LINC00559 as a biomarker in the preparation of lung adenocarcinoma prognosis detection reagents. Background technique [0002] The mortality rate of lung cancer is extremely high, and it is one of the main causes of related deaths caused by malignant tumors worldwide, accounting for 28% of cancer deaths in men and 26% in women. At the beginning of the 21st century, the World Health Organization announced the prevalence of different types of lung cancer. Among them, lung adenocarcinoma ranked first, with an incidence rate of 31.5%, followed by lung squamous cell carcinoma, with an incidence rate of 29.4%, and small cell lung cancer. The rate was 17.8%, and the incidence rate of large cell lung cancer was 9.2%. The onset of lung adenocarcinoma is relatively hidden, and the metastasis is early. Patients often have metast...

Claims

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Application Information

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IPC IPC(8): C12Q1/6886
CPCC12Q1/6886C12Q2600/118C12Q2600/158C12Q2600/166C12Q2600/178
Inventor 陶永光毛超王敏刘双
Owner XIANGYA HOSPITAL CENT SOUTH UNIV