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A technology of NK cells and cell lines, applied in the field of cell lines, can solve the problems of difficulty in purifying NK cells, and achieve the effect of strong anti-tumor activity
Active Publication Date: 2018-06-29
INST OF GENETICS & DEVELOPMENTAL BIOLOGY CHINESE ACAD OF SCI
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However, there are certain difficulties in the purifi
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Embodiment 1
[0031] 1. Establishment of M296 cell line
[0032] 1.1 The source of the specimen:
[0033] 2ml of peripheral blood was taken from a female patient with chronic active EBV infection, anticoagulated with heparin. The informed consent was signed with the consent of the patients and their families.
[0034] 1.2 Isolation of peripheral blood mononuclear cells:
[0035] Aseptically extract 2ml of peripheral blood from the patient, and conduct density gradient centrifugation with lymphocyte separation medium at 2000 rpm for 20 minutes at a room temperature of 20 degrees. Aspirate the mononuclear cells in the middle layer, wash twice with PBS, 1500 rpm, 10 minutes, room temperature 20 degrees. Add fresh and complete 1640 culture medium, divide 2ml of the cell suspension into the wells of a 24-well culture plate, culture in a 37°C, 5% CO2 incubator, and add 50-700U of recombinant human IL-2.
[0036] 1.3 Cell culture and establishment
[0037] After the cells were cultured for 72...
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Abstract
The invention relates to the cell line, and specifically relates to a NK cell line of the human. The conservation number is CGMCC No. 14891. The NK cell line of the human provided by the invention canbe passaged for a long time and greatly amplified, and has stronger anti-tumor activity, and provides a new experimental model for deeply development chronic activity EBV infectious disease mechanismresearch, screening chronic activity EBV infection, treatment medicine of EBV related lymphocytosis and the like, natural killer cell anti-tumor action mechanism and clinic adoptive immunity treatment for a researcher.
Description
technical field [0001] The present invention relates to cell lines, in particular to a human NK cell line. Background technique [0002] Natural killer cells (NK cells) are important immune cells in the body, which have anti-tumor, anti-viral infection and immune regulation functions. [0003] NK cells have long been considered an excellent weapon for killing cancer cells and treating infections. However, there are certain difficulties in the purification and large-scale expansion of NK cells in vitro. Therefore, scientists try to establish cell lines and use allogeneic NK cells for tumor biotherapy. Foreign scientists have successively established HANK, KHYG-1, NK-92, NKL, PLT, MOTN, IMC-1, NK-YS, KAI3, SNK -1, SNK6, YT, NK3.3, etc., as well as transgenic NK cell lines such as NK-92MI, IL-2 / NKL, IL-2 / NK3.3 obtained by stably transfecting IL-2. In particular, the establishment of NK-92 has promoted its application in tumor adoptive immunotherapy, and provided powerful res...
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IPC IPC(8): C12N5/0783C12Q1/02A61K35/17A61P35/00
CPCA61K35/17C12N5/0646G01N33/5011
Inventor 段子渊许崇凤谢正德张庆勋
Owner INST OF GENETICS & DEVELOPMENTAL BIOLOGY CHINESE ACAD OF SCI
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