The invention discloses a method for establishing an EBV (Epstein-
Barr Virus) infected artificial
respiratory tract epithelium model. The method comprises the following steps: (1) digesting nasopharyngeal mucosa tissues by using
Dispase II, performing beating into single cells, and performing centrifuging to remove supernate, so as to obtain
cell precipitate; (2) suspending and culturing the cellprecipitate by using an epithelial
cell culture medium; (3) performing digesting into single cells, planting the single cells in a small chamber above the 24-hole support membrane, and performing culturing until the support membrane is overgrown; (4) completely removing the culture solution above the support membrane, and continuously culturing for 2-3 weeks; and (5) when large-area
cilium swinging is observed, adding Akata cells to carry out
cell contact mediated EBV infection, and when successful infection, obtaining the artificial
respiratory tract epithelium model infected with the EBV. According to the establishing method, nasopharyngeal mucosa tissue is taken as a material part, nasopharyngeal epithelial cells can be differentiated into completely polarized pseudo-multilayer
respiratory tract epithelial tissue through gas-
liquid interface culture, and the nasopharyngeal
epithelial tissue is completely simulated by basal layer cells, secretory cuppy cells and swinging
cilium cells.