A kind of iron crabapple extract and its preparation method and its application in the preparation and prevention of ebv virus infection medicine
A technology of extract and Thornweed, applied in the field of active ingredients of traditional Chinese medicine and medicine, can solve the problems of no compound inhibiting virus lysis and replication, no anti-EBV virus lysis and replication activity, etc., and achieve the effect of low host cell toxicity
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Embodiment 1
[0048] The method for preparing compound 1~19 from Tiehaitang (Euphorbia milii):
[0049]The aerial part (3kg) of Euphorbia milii was dried in the sun, pulverized, soaked and extracted with 80% acetone water for 3 times, each time for 24 hours, and the concentrated solutions were combined to obtain a crude extract (400g), extracted with ethyl acetate, Ethyl acetate (180g) was directly subjected to silica gel column chromatography (1500g, 200-300 mesh, 7.0×100cm), cyclohexane-ethyl acetate (0:100, 10:90, 30:70, 50:50, 70:30, 80:20, 90:10, 100:0, V / V) gradient elution, each 500mL was collected, and the same fraction was combined for TLC detection, and a total of 3 fractions Fr.A-C were obtained. Part of Fr.B (50g) was eluted with silica gel column chromatography (250g, 200-300 mesh, 4.5×60cm) with dichloromethane-ethyl acetate (200:1, V / V) to obtain six fractions Fr. B1-6. Fr.B1 (300mg) was eluted with SephadexLH-20 with dichloromethane-methanol (1:1) to give compounds 17 (3mg...
Embodiment 2
[0052] According to the method of Example 1, compounds 1-19 were routinely added with water for injection, finely filtered, potted and sterilized to make an injection.
Embodiment 3
[0054] According to the method of Example 1, compounds 1-19 were dissolved in sterile water for injection, stirred to dissolve, and filtered with a sterile suction filter funnel. Then aseptically finely filtered, divided into 2 ampoules, freeze-dried at low temperature, and aseptically melt-sealed to obtain a powder injection.
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