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EBV capture probe and method for acquiring EBV genome sequence information in sample

A genome sequence and capture probe technology, applied in biochemical equipment and methods, recombinant DNA technology, microbial measurement/testing, etc., can solve the problems of low probe capture efficiency, low probe coverage, no special design, etc. , to achieve the effect of avoiding non-specific data, high capture efficiency and uniform coverage

Inactive Publication Date: 2018-03-30
SUN YAT SEN UNIV CANCER CENT
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  • Abstract
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  • Application Information

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Problems solved by technology

[0003] At present, the capture probe products designed for EBV have some common shortcomings: in various diseases related to EBV, such as lymphoma, nasopharyngeal carcinoma, gastric cancer, etc., and in EBV-containing cell lines (C666, akata, B958, etc.) , the probe capture efficiency is low, the probe coverage is low, the sequencing data coverage is not uniform, and some EBV genome regions are not covered
The main reason for the above shortcomings is that the complexity and heterogeneity of the target sequence may not be fully considered when designing probes for these products, and more specific probes are not specially designed to solve the problem for some difficult-to-capture regions

Method used

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  • EBV capture probe and method for acquiring EBV genome sequence information in sample
  • EBV capture probe and method for acquiring EBV genome sequence information in sample
  • EBV capture probe and method for acquiring EBV genome sequence information in sample

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Embodiment 1

[0032] (1) Based on the principle of nucleic acid molecular base complementary hybridization, according to the EBV genome reference sequence, design specific

[0033] sex RNA probe

[0034] The probe is based on the public database and the EBV genome information obtained by our research team as a reference sequence, based on the principle of complementary hybridization of nucleic acid molecule bases, and adopts tile design. The nucleotide sequence of the probe involved in the present invention is as SEQID NO:1 ~As shown in SEQ ID NO: 48, the recommended sequencing data volume for each sample is 183.646Mbp. The RNA probe we designed has high fault tolerance and strong binding ability, and can achieve strong capture efficiency in a variety of EBV-related diseases.

[0035] (2) Genomic library establishment: The library construction kit is as follows: SureSelect XT Library Prep Kit ILMcat#5500-0133

[0036] SureSelect Target Enrichment System 1-800-227-9770

[0037] Herculase ...

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Abstract

The invention provides an EBV capture probe and a method for acquiring EBV genome sequence information in a sample. The capture probe designed by the invention has the following advantages: the capture efficiency on various EBV infection-associated diseases such as lymphoma, nasopharynx cancer and gastric cancer as well as EBV-containing cell lines (C666, Raji and snu179) is relatively high, the coverage degree is close to 100 percent and the sequencing data coverage is uniform, so that the chip can capture the information of the EVB genome well and has important significance in deep researchon the virus.

Description

technical field [0001] The invention relates to an EBV capture probe and a method for obtaining EBV genome sequence information in a sample. Background technique [0002] Epstein-Barr virus (EBV), also known as human herpesvirus 4 (Human herpesvirus 4, HHV-4), commonly infects the global population. Studies have shown that EBV is closely related to nasopharyngeal carcinoma, lymphoma, gastric cancer and other tumors, mononucleosis and autoimmune diseases, suggesting that there may be specific pathogenic subtypes of EBV, and the specific mechanism has not yet been clarified. Therefore, the study of the EBV genome will help to reveal the pathogenic mechanism of EBV infection-induced diseases, and to screen out more effective clinical diagnosis and treatment-related virus molecular markers. [0003] The current capture probe products designed for EBV have some common shortcomings: in various diseases related to EBV, such as lymphoma, nasopharyngeal carcinoma, gastric cancer, et...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/70C12Q1/6869C12Q1/6806C12N15/11
CPCC12Q1/6806C12Q1/6869C12Q1/705C12Q2600/166
Inventor 曾益新贝锦新冯丽娜刘稳升左晓宇郭允苗彭柔君
Owner SUN YAT SEN UNIV CANCER CENT
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