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Method for extracting purified active lentinan from leftover materials of edible fungi

A technology for mushroom polysaccharide and leftover material is applied in the field of extracting and purifying active mushroom polysaccharide, which can solve the problems of affecting the quality and quality of purified active mushroom polysaccharide, loss of purified active mushroom polysaccharide, lack of a better extraction method, etc., and achieves prevention of loss and simple equipment. , to ensure the effect of purity

Inactive Publication Date: 2018-07-06
广州市上品荟科技发展有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Aiming at the deficiencies of the prior art, the present invention provides a method for extracting and purifying active mushroom polysaccharides from edible fungus leftovers, which solves the problem of not having a better extraction method, which may easily lead to the loss of purified active mushroom polysaccharides during extraction, or There are many impurities in the purified active mushroom polysaccharide, which affects the quality and quality of the purified active mushroom polysaccharide

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Step 1. Material preparation: select the leftovers of edible fungi, and remove the leftovers of edible fungi with damage, corrosion, etc.;

[0028] Step 2, material preparation: weigh 2g of the prepared edible fungus leftovers, cut the 2g edible fungi leftovers into small pieces, add a certain weight of water, and then homogenize with a tissue masher;

[0029] Step 3. Heating the homogeneous solution: take 18mL of the homogeneous solution and put it into a 0.9L beaker, then add 27mL of distilled water, heat to boil, then boil on low heat for 0.9 hours;

[0030] Step 4. Filtration: After heating, filter the liquid in the cup with 8 layers of gauze to remove the residue, and transfer the supernatant to another beaker;

[0031] Step 5. Concentrate the supernatant: pour the supernatant into a round-bottomed flask, concentrate on a rotary concentrator, and stop when the volume of the concentrate reaches 95mL;

[0032] Step 6, concentrate centrifugation: centrifuge the conce...

Embodiment 2

[0038] Step 1. Material preparation: select the leftovers of edible fungi, and remove the leftovers of edible fungi with damage, corrosion, etc.;

[0039] Step 2, material preparation: weigh 2g of the prepared edible fungus leftovers, cut the 2g edible fungi leftovers into small pieces, add a certain weight of water, and then homogenize with a tissue masher;

[0040] Step 3. Heating the homogeneous solution: Take 20mL of the homogeneous solution and put it into a 1L beaker, then add 30mL of distilled water, heat to boil, then boil on low heat for 1 hour;

[0041] Step 4. Filtration: After heating, filter the liquid in the cup with 8 layers of gauze to remove the residue, and transfer the supernatant to another beaker;

[0042] Step 5. Concentrate the supernatant: pour the supernatant into a round-bottomed flask, concentrate on a rotary concentrator, and stop when the volume of the concentrate reaches 100mL;

[0043] Step 6, concentrate centrifugation: centrifuge the concentra...

Embodiment 3

[0049] Step 1. Material preparation: select the leftovers of edible fungi, and remove the leftovers of edible fungi with damage, corrosion, etc.;

[0050] Step 2, material preparation: weigh 2g of the prepared edible fungus leftovers, cut the 2g edible fungi leftovers into small pieces, add a certain weight of water, and then homogenize with a tissue masher;

[0051]Step 3. Heating the homogeneous solution: Take 22mL of the homogeneous solution and put it into a 1.1L beaker, then add 33mL of distilled water, heat to boil, then boil on low heat for 1.1 hours;

[0052] Step 4. Filtration: After heating, filter the liquid in the cup with 8 layers of gauze to remove the residue, and transfer the supernatant to another beaker;

[0053] Step 5. Concentrate the supernatant: pour the supernatant into a round-bottomed flask, concentrate on a rotary concentrator, and stop when the volume of the concentrate reaches 105 mL;

[0054] Step 6, concentrate centrifugation: centrifuge the conc...

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PUM

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Abstract

The invention discloses a method for extracting purified active lentinan from leftover materials of edible fungi. The method comprises the following specific steps of: material preparation, material blending, homogeneous-liquid heating, filtering, supernatant-liquor concentration, concentrated-liquor centrifugation, supernatant-liquor stirring and standing, mixed-liquor centrifugation, aqueous-phase treatment and precipitate drying. The method for extracting the purified active lentinan from the leftover materials of the edible fungi has the beneficial effects that the problem of relatively single mode for extracting the purified active lentinan from the leftover materials of the edible fungi currently is solved, a better extraction mode is provided, the easy loss of the purified active lentinan in extraction is prevented, the purity of the purified active lentinan can be better ensured, the quality of the purified active lentinan is ensured, and the defects hard to overcome are overcome; compared with the traditional method, the microwave-assisted extraction method has the characteristics of simple equipment, fastness, high efficiency and safety and the like.

Description

technical field [0001] The invention relates to the technical field of extracting purified active mushroom polysaccharides from leftovers of edible fungi, in particular to a method for extracting and purifying active mushroom polysaccharides from leftovers of edible mushrooms. Background technique [0002] Mushroom polysaccharides have no immune-promoting effect on normal organisms, but can improve the immune response of tumor-bearing or infected organisms. Its preparation has no direct anti-cancer effect in the animal screening test, but it can obviously promote the transformation of lymphocyte culture in vitro. It has been found that injection of anti-lymphocyte serum into thymectomized animals can weaken the anti-tumor activity of mushroom polysaccharide, and its effect can also be weakened by macrophage inhibitors carrageenan and silica gel. Therefore, mushroom polysaccharide is a special immune adjuvant directed by thymus-dependent T cells and participated by macrophag...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00
CPCC08B37/0003
Inventor 范昇
Owner 广州市上品荟科技发展有限公司