A strain of Bacillus subtilis, bacterial agent containing the bacterium and its application, method and kit for degrading vomitoxin

Abstract

The invention relates to the field of microorganisms, and discloses a bacillus subtilis, a bacterial agent containing the bacterium and its application, a method for degrading vomitoxin and a kit. Specifically, the present invention provides a strain of Bacillus subtilis, the preservation number of which is CGMCC NO.13139. The Bacillus subtilis provided by the present invention can efficiently and rapidly degrade the vomitoxin in the grain, oil and / or feed, especially, even when the content of the vomitoxin in the grain, oil and / or feed is as high as 50 ppm or more, the Bacillus subtilis can also efficiently and rapidly Degrade vomitoxin without affecting the palatability of grain, oil and / or feed; at the same time, it is safe to add the Bacillus subtilis to grain, oil and / or feed contaminated by vomitoxin. Therefore, the Bacillus subtilis has a good application prospect.

Description

technical field [0001] The invention relates to the field of microorganisms, in particular, to a strain of Bacillus subtilis, a bacterial agent containing the bacteria and its application, and a method and kit for degrading vomitoxin. Background technique [0002] Vomitoxin (vomitoxin), also known as deoxynivalenol (DON), the chemical name is 3α,7α,15-trihydroxyfusarium-9-en-8-one, because it can cause swine It is named for the trichothecenes produced by Fusarium graminearum and Fusarium culmorum infecting wheat, barley, oats, corn and other grains. All over the world, DON is one of the main contaminating mycotoxins in grain, feed and food, seriously affecting the health of humans and livestock. After humans and animals ingest food contaminated with DON, it will cause acute poisoning symptoms such as anorexia, vomiting, diarrhea, fever, unsteady standing, unresponsiveness, and in severe cases, damage the hematopoietic system and cause death. General attention. [0003] Th...

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Problems solved by technology

Patent application CN103243047A discloses a strain of Bacillus subtilis that efficiently degrades deoxynivalenol and its application. 900 μL of Bacillus subtilis ANSB471 fermentation broth is reacted with 100 μL of deoxynivalenol (100 μg / ml), and the degradation rate of deoxynivalenol for 2 hours is 25 %, the degradation rate of vomitoxin in 24 hours of reaction is 56%, and the degradation rate of vomitoxin in 48 hours of reaction is 80%, and the degradation rate still needs to be improved

[0005] In addition, most of the existing biological methods for degrading vomitoxin are carried out under mild conditions (such as a temperature of 25-37°C and not exceeding 40°C, and a pH of about 7), however, under higher temperature loads (such as During transport in containers or during feed pelleting) or harsh acidic and alkaline conditions, there is no better solution, which limits the scope of application of biological methods in the degradation of vomitoxin

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