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Seed endophytes across cultivars and species, associated compositions, and methods of use thereof

A technology of endophytic bacteria and seeds, which is applied in the field of improving plant characteristics and cultivation compositions, and can solve the problems of lack of recognition of GM traits in wheat

Inactive Publication Date: 2018-07-10
靛蓝股份公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition to their long-term development and regulatory timelines, public concerns about GM crops and synthetic chemicals have called into question their use in many key crops and countries, leading to a complete lack of acceptance of GM traits in wheat and rejection of the European market. GM crops and many synthetic chemicals

Method used

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  • Seed endophytes across cultivars and species, associated compositions, and methods of use thereof
  • Seed endophytes across cultivars and species, associated compositions, and methods of use thereof
  • Seed endophytes across cultivars and species, associated compositions, and methods of use thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0480] Example 1: Culture-Independent Analysis of Microbial Taxa in Agriculture-Related Seed Populations Based on High-Throughput Sequencing of Marker Genes

[0481] Example description

[0482] Using high-throughput marker gene sequencing on several crops and multiple seed varieties to identify microbial taxa found in agriculturally relevant populations.

[0483] Experiment description

[0484] To identify core (ie, ubiquitous) microbial taxa in seeds, we used high-throughput sequencing of marker genes for bacteria, archaea, and fungi.

[0485] cereal

[0486] 2 inbred, 10 landrace, 4 teosinte corn seeds and 4 modern and 4 wild wheat seeds were obtained. Additions are categorized as landraces, wild and inbred based on an assessment of their improvement status. To extract microbial DNA, the seeds are first sterilized in one of four different ways. Some seeds were surface sterilized with 95% ethanol to reduce surface contaminating microorganisms, followed by rinsing in wat...

Embodiment 2

[0527] Example 2 - Identification and Characterization of Cultivable Bacterial and Fungal Endophytes Belonging to the Core OTUs

[0528] To better understand the role of endophytic microorganisms derived from core seeds in improving the vigor, general health, and stress resistance of agricultural plants, we pioneered a systematic screen for seed extraction from commercially significant agricultural plants. and tissues to isolate and characterize endophytic microorganisms.

[0529] Seeds were obtained from various types of cereals, fruits, vegetables, grasses, oilseeds and other seeds and screened for culturable microorganisms as described below. Cultivable microorganisms belonging to the same OTU as the core OTUs described in Tables 1 and 2 (ie, SYM strains) were isolated and identified.

[0530] Isolation of bacteria and fungi from inside the seeds

[0531] Internal isolation of fungi and bacteria (including endophytes) from surface sterilized seeds is performed using techn...

Embodiment 3

[0632] Example 3: Synthetic compositions comprising plant seeds and a single or multiple endophyte strains confer benefits on agricultural plants

[0633] This example describes the ability of a synthetic composition comprising a plant seed and a single endophyte strain or a plurality of endophyte strains described herein to confer beneficial traits on a host plant. This example describes, inter alia, the effects of endophytes (e.g., the bacterial and fungal endophytes described herein) on various host plants including, but not limited to, dicots (e.g., soybeans, peanuts) and monocots (e.g., , corn, soybean, wheat, cotton, sorghum) and combinations thereof) the ability to confer beneficial traits. Endophyte-inoculated seeds (e.g., the described seeds) to test whether one or more endophytes confer increased tolerance to one or more stresses. Growth tests were performed using growth assays (eg, germination assays and seedling root viability assays) on sterile filter paper. In...

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Abstract

Materials and methods for improving plant traits and for providing plant fitness benefits are provided. In some embodiments, the materials, and methods employing the same, can comprise endophytes.

Description

[0001] Cross-references to related applications [0002] This application requires international application No. PCT / US2015 / 038187 filed on June 26, 2015, U.S. Provisional Application No. 62 / 156,021 filed on May 1, 2015, and U.S. Provisional Application No. filed on May 1, 2015. .62 / 156,028, U.S. Provisional Application No. 62 / 098,296 filed on December 30, 2014, U.S. Provisional Application No. 62 / 098,298 filed on December 30, 2014, U.S. Provisional Application filed on December 30, 2014 No. 62 / 098,299, U.S. Provisional Application No. 62 / 098,302 filed on December 30, 2014, and U.S. Provisional Application No. 62 / 098,304 filed on December 30, 2014, each of which is incorporated by reference in its entirety. are incorporated into this article. [0003] sequence list [0004] This application contains the sequence listing submitted via EFS-Web and incorporated herein by reference in its entirety. The ASCII copy created on December 30, 2015 is named 10035_Final_ST25.txt and i...

Claims

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Application Information

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IPC IPC(8): A01C1/00A01G22/40A01G7/06A01H1/04A01N63/20A01N63/22A01N63/27A01N63/30
CPCA01C1/06A01G7/06A01H1/04C05F11/08A01N37/46A01N63/30Y02W30/40A01N63/27A01N63/22A01N63/20
Inventor K·V·安布罗斯N·A·彼林格斯S·迪乔诺维克R·B·弗拉维尔T·A·古利克D·M·约翰斯顿J·W·莱夫S·M·利瓦J·莱福德G·冯马尔特扎恩L·M·马尔克斯Y·A·米利特C·萨道斯基P·萨马约亚G·V·托莱多D·R·韦斯曼张雪程
Owner 靛蓝股份公司
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