A kind of rice smut effector gene smut_5844 and its application

Abstract

The invention provides a rice kernel smut fungus effector gene Smut_5844 and application thereof. The nucleotide sequence of the gene is shown as SEQ ID NO:1; and the amino acid sequence of a proteincoded by the gene is shown as SEQ ID NO:2. By cloning the rice kernel smut fungus effector gene and analyzing the function, a pesticide molecular target can be designed; the receptor protein gene of the effector protein in host cells of rice and the like can also be knocked out or mutated to obtain a persistently disease-resistant variety; the invention can help establish a molecular assay systemfor the pathogenic mutation of the natural population of smut fungi, the distribution of the smut fungus effector gene in the field natural population is studied, and the composition and mutation characteristics of microspecies in the smut fungus population are disclosed; and the invention also contributes to the evaluation of the disease resistance of rice varieties and variety distribution and rotation, so that the occurrence of rice kernel smut fungui can be effectively controlled.

Description

technical field [0001] The invention belongs to the technical field of genetic engineering, in particular to a rice smut effector gene Smut_5844 and its application. Background technique [0002] Rice smut is a soil-borne fungal disease caused by Tilletia horrida, which is distributed in many rice regions in Asia, America and Africa. Smut has a wide range of hosts, including rice, corn, sugarcane, sorghum, barley and other crops. The ability of smut to infect a variety of crops is related to the secreted effector molecules, which can regulate the host's innate immunity and enhance self-infection. It is now generally accepted that these effector molecules are key pathogenic factors that enhance parasitic infection. [0003] By secreting effector molecules, the smut fungus tightly binds to its host and manipulates the host cells to change the process of the host plant. In the process of interaction between plant pathogens, effector molecules are the main pathogenic factors....

AI Technical Summary

Problems solved by technology

However, AvrLm1 has only one cysteine ​​residue, and considering that all the extraplastid effectors described so far are cysteine-rich proteins, AvrLm1 is likely to be transported into the host cell

Although it is clear that they have avirulent activity, the mechanism of AvrLm recognition, the corresponding Rlm resistance genes and their sites of action remain unclear

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