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Application of Tamoxifen in the Preparation of Drugs for Protecting Retinal Photoreceptor Cells

A technology of tamoxifen and photoreceptor cells, which is applied in the field of medicine, can solve problems such as weight loss, disturbed menstrual cycle, and increased incidence of uterine cancer, and achieve the effects of increasing survival rate, improving electroretinogram waveform, and reducing apoptosis rate

Active Publication Date: 2019-11-05
王晓琳
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Tamoxifen has certain side effects, such as disrupting the menstrual cycle, losing weight, and increasing the risk of uterine cancer, stroke, or pulmonary embolism
There is no report on the improvement of subretinal hemorrhage by tamoxifen

Method used

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  • Application of Tamoxifen in the Preparation of Drugs for Protecting Retinal Photoreceptor Cells
  • Application of Tamoxifen in the Preparation of Drugs for Protecting Retinal Photoreceptor Cells
  • Application of Tamoxifen in the Preparation of Drugs for Protecting Retinal Photoreceptor Cells

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] C57BL / 6 mice, 17 2-month-old mice, were divided into 2 groups: 10 mice in the experimental group, 5 male mice and 5 female mice; 7 mice in the control group, 4 male mice and 3 female mice.

[0041] The treatment group had a normal diet, and the rats were given intragastric administration of tamoxifen at a dose of 400 mg per kilogram of body weight, once every 2 days,

[0042] The control group had a normal diet, and the mice were given the same amount of excipients by gavage, once every 2 days.

[0043] On the 4th day, the autologous blood from the tail of the mouse was injected under the retina of the right eye, and after another 4 days, the right eye was taken and embedded in agar for sectioning for testing. The testing included:

[0044] 1: TUNEL detection

[0045] DAPI (4,6-Diamidino-2-phenylindole, 4',6-diamidino-2-phenylindole) was used to stain cell nuclei, and Roche's apoptosis detection kit was used to detect cell apoptosis (principle: terminal Deoxynucleotid...

Embodiment 2

[0055] Four C57BL / 6 mice, 2 months old, were divided into 2 groups; 2 mice in the experimental group, 1 male mouse and 1 female mouse; 2 mice in the control group, 1 male mouse and 1 female mouse.

[0056] When the mice were not treated, detect the dark adaptation electroretinogram (scotopicelectroretinogram, ERG) of the left and right eyes of the mice, and the light stimulation intensity of ERG: 10cd.s / m 2 .

[0057] Thereafter, the treatment group took a normal diet and administered tamoxifen to the mice at a dose of 80 mg per kilogram of body weight once a day.

[0058] The control group had a normal diet, and the mice were gavaged with the same amount of excipients once a day.

[0059] After 6 days, detect the ERG of the left and right eyes of the mouse again, and the intensity of light stimulation: 10cd.s / m 2 .

[0060] The results showed that no matter male or female rats, after administration of tamoxifen, ERG changes (the arrow indicates the change), which should be...

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Abstract

The invention relates to the technical field of medicaments, in particular to application of tamoxifen in preparation of a medicament for protecting retina photoreceptor cells. According to the application, mouse models with subretinal hemorrhage caused by an autologous blood injection mode are adopted; research shows that the survival rate of the retina photoreceptor cells which are in contact with blood can be improved, the apoptosis rate of the retina photoreceptor cells can be reduced, and the thickness of a retina outer nuclear layer can be maintained by giving the tamoxifen. A protectingeffect on the retina photoreceptor cells can be applied in a protective manner, namely, the tamoxifen is used when new vessels do not burst. Based on experimental results, the protecting effect can also be achieved on surviving retina photoreceptor cells in a retina even after bleeding. The tamoxifen can change an electroretinogram waveform of a normal mouse, which is one of the mechanisms for protecting the retina photoreceptor cells.

Description

technical field [0001] The invention relates to the technical field of medicine, in particular to the application of tamoxifen or its metabolites in human body in the preparation of drugs for protecting retinal photoreceptor cells. Background technique [0002] Subretinal hemorrhage is a clinical phenomenon, not an independent eye disease, but a type of fundus hemorrhage. Subretinal hemorrhage occurs in a variety of diseases, such as age-related macular degeneration (AMD) or polypoidal choroidal vasculopathy (PCV). Fundus hemorrhage includes subretinal hemorrhage, vitreous hemorrhage, and retinal hemorrhage. Vitreous hemorrhage is often caused by rupture of retinal neovascularization, which is more common in advanced diabetic retinopathy and retinal branch or central vein occlusion. Retinal hemorrhage is more common in early diabetic retinopathy and retinal branch or central vein occlusion. [0003] Subretinal hemorrhages damage the photoreceptor cells in the retina, impa...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K31/138A61P27/02
CPCA61K31/138
Inventor 不公告发明人
Owner 王晓琳
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