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Detection method of electrochemical fingerprints of artificial bos taurus domesticus gmelin

A technology of artificial bezoar and fingerprints, which is applied in the detection field of electrochemical fingerprints, can solve the problems that qualitative analysis cannot be used for quantitative detection, etc., and achieve the effect of high accuracy, good reproducibility and low price

Inactive Publication Date: 2018-07-27
吉林省吴太感康药业有限公司 +1
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  • Claims
  • Application Information

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Problems solved by technology

However, the current research results show that this method can only be used for qualitative analysis but not for quantitative detection

Method used

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  • Detection method of electrochemical fingerprints of artificial bos taurus domesticus gmelin
  • Detection method of electrochemical fingerprints of artificial bos taurus domesticus gmelin
  • Detection method of electrochemical fingerprints of artificial bos taurus domesticus gmelin

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0062] Embodiment 1 establishes the electrochemical fingerprint of artificial bezoar chemical composition

[0063] Taking 6 batches of artificial bezoar from a regular manufacturer in Hunan, all of them were qualified products by liquid chromatography, and their electrochemical fingerprints were established as standard samples.

[0064] Instruments used: CHI600E electrochemical workstation, HH.S214 electric constant temperature water bath, 85-1 magnetic stirrer, ZNHW-IV intelligent constant temperature temperature controller, 232 saturated calomel electrode, 213 platinum electrode;

[0065] Detection method: The electrochemical oscillation reaction is carried out in a jacketed reactor with continuous stirring. Turn on the super constant temperature water bath to adjust the temperature of the water bath to (310±0.05)K and then keep the temperature. Add 0.5g artificial bezoar, 10mL distilled water, 2mL 98% Concentrated sulfuric acid solution, 2mL malonic acid solution (0.45mol / L...

Embodiment 2

[0067] Embodiment 2 detection sample

[0068] Use above-mentioned measuring condition identical with standard sample, measure the artificial bezoar of different origin, manufacturer (Hebei, Anhui, Guangxi, Hunan) respectively, the result of its electrochemical fingerprint spectrum is as follows figure 2 shown. Judging from the entire electrochemical fingerprint, the E-t curves of different origins and manufacturers are obviously different, which can be distinguished by naked eyes. The RSD of various characteristic parameters of the measured electrochemical fingerprint spectrum is more than 10%, which shows that the method can be used for the qualitative detection of artificial bezoar.

[0069] The electrochemical fingerprint method for identifying artificial bezoar of the present invention is stable for various characteristic parameters of the electrochemical fingerprint of the chemical composition of a specific sample. When identifying, it is only necessary to compare the ...

Embodiment 3

[0071] Embodiment 3 reproducibility investigation

[0072] Take 6 samples of artificial bezoar to be tested, and measure their electrochemical fingerprints. The RSD values ​​of the highest potential, induction time, oscillation period, and oscillation life of the electrochemical fingerprints are: 2.37%, 1.54%, 2.15%, 0.97%, and the RSD of each characteristic parameter is less than 3%, indicating that the method has good reproducibility.

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Abstract

The invention relates to the technical field of quality control of traditional Chinese medicine, in particular to a detection method of electrochemical fingerprints of artificial bos taurus domesticusgmelin. The detection method comprises steps as follows: the artificial bos taurus domesticus gmelin is added to an oscillating reaction system at constant temperature and then is subjected to electrochemical detection in a stirring state by adopting a platinum electrode as an indicator electrode and a saturated calomel electrode as a reference electrode, an E-t curve is drawn, and the electrochemical fingerprints of the artificial bos taurus domesticus gmelin are obtained; in terms of g / mL, a massic volume ratio of the artificial bos taurus domesticus gmelin to the oscillating reaction system is (1-4):100. The electrochemical fingerprints reflected by all chemical components in the artificial bos taurus domesticus gmelin are obtained, the operating method is fast and convenient, consumable cost is relatively low, repeatability and accuracy are high, identification effect is quite good, and the method can be applied to quantitative determination; purposes of identifying the artificialbos taurus domesticus gmelin as traditional Chinese medicine, optimally selecting production places and controlling quality can be achieved, information is reliable, and the method is suitable for being widely popularized and applied to the technical field of safety detection of the artificial bos taurus domesticus gmelin.

Description

technical field [0001] The invention relates to the technical field of quality control of traditional Chinese medicines, in particular to a method for detecting electrochemical fingerprints of artificial bezoar. Background technique [0002] Bezoar (Bos taurus domesticus Gmelin) is the dried gallstone of bovid cattle. Traditional Chinese medicine believes that bezoar is fragrant, slightly bitter and then sweet, and cool in nature. It can be used for antipyretic, detoxification and calming convulsions. Take it orally to treat high fever, coma, mania, infantile convulsions, convulsions and other diseases. Because natural bezoar is very precious, the international price is higher than gold, so most of the products are artificial bezoar. Artificial bezoar is made from bovine bile powder, cholic acid, hyodeoxycholic acid, taurine, bilirubin, cholesterol, trace elements, etc. Due to its complex composition, the traditional identification method is cumbersome. The four-step chem...

Claims

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Application Information

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IPC IPC(8): G01N27/26G01N27/30
Inventor 邢福涛
Owner 吉林省吴太感康药业有限公司
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