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Application of flavonoid GL-V9 in preparation of anti-leukemia drugs

A technology of flavonoids and GL-V9, applied in the field of application of flavonoids GL-V9 in the preparation of anti-leukemia drugs

Inactive Publication Date: 2018-08-03
CHINA PHARM UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But there is no literature report that it has anti-leukemia effect

Method used

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  • Application of flavonoid GL-V9 in preparation of anti-leukemia drugs
  • Application of flavonoid GL-V9 in preparation of anti-leukemia drugs
  • Application of flavonoid GL-V9 in preparation of anti-leukemia drugs

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 3

[0077] Example 3: Effect of GL-V9-induced DC-like cells produced by AML cells on secreted factors after co-culture with T cells.

[0078] 1. Experimental materials

[0079] 1.1 Reagents

[0080] (1) Drugs

[0081] GL-V9(C 24 h 27 o 5 N, molecular weight: 409.47) was purchased from China Pharmaceutical University, a light yellow powder with a purity greater than 99%. Before use, the drug powder was prepared into a mother solution with a concentration of 0.02M with dimethyl sulfoxide (DMSO), and stored at -20°C. Prepare the required concentration with RPMI-1640 culture medium containing 10% fetal bovine serum before use

[0082] (2) Cell culture reagents

[0083] Culture medium: RPMI-1640 medium, purchased from GIBCO, USA. Take 10.39g of RPMI-1640 powder and dissolve it in 1000ml sterile three-distilled water, and add 2.0g NaHCO 3 , adjust the pH value to 7.0 with 1M hydrochloric acid, filter and sterilize with a cylindrical filter, pack in aliquots, and store in a refri...

Embodiment 1

[0110]Take 10g of GL-V9, add appropriate auxiliary materials for injection (including freeze-dried powder injection and sterile subpackaged dry powder injection), and prepare 1000ml of injection according to the injection (including freeze-dried powder injection and sterile subpackaged dry powder injection) process.

Embodiment 2

[0112] Take 10g of GL-V9, add appropriate excipients for tablets (including sustained and controlled release tablets, matrix tablets, coated tablets, dispersible tablets, etc.), press the tablet (including sustained and controlled release tablets, matrix tablets, coated tablets, dispersible tablets, etc.) ) process is prepared into 1000 tablets.

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Abstract

The invention belongs to the field of preparation of drugs, and discloses application of flavonoid GL-V9 in preparation of anti-leukemia drugs. The flavonoid GL-V9 can guide acute myeloid leukaemia cells to become into dendritic cells, and can be used for preparing anti-leukemia drugs.

Description

technical field [0001] The invention belongs to the field of pharmacy and relates to the application of flavonoid compound GL-V9 in the preparation of anti-leukemia drugs. Background technique [0002] Compound GL-V9 is a derivative of flavonoids with structural optimization of wogonin. It is synthesized from the natural product wogonin through two-step reactions. First, it is synthesized in acetone and K 2 CO 3 Under the above reaction conditions, the phenolic hydroxyl group on the C7 position of wogonin was replaced by 1,4-dibromobutane, and then the bromine in the product of the previous step was replaced by pyrrolidine to finally obtain GL-V9. [0003] Previous studies have shown that GL-V9 has a wide range of anti-tumor effects, including promoting the apoptosis of gastric cancer cells MGC-803 and human liver cancer cells HepG2, inhibiting the invasion and metastasis of human breast cancer cells MDA-MB-231 and MCF-7, improving DSS-induced colitis and inhibition of col...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/4025A61P35/02
CPCA61K31/4025A61P35/02
Inventor 郭青龙魏立彬惠慧卢娜赵丽赵凯周煜新
Owner CHINA PHARM UNIV
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