Unlock instant, AI-driven research and patent intelligence for your innovation.

Preparation method and application of anglerfish bone antitumor decapeptide

An anti-tumor, anglerfish technology, applied in anti-tumor drugs, medical preparations containing active ingredients, peptides, etc., can solve problems such as pollution of the environment, waste of resources, etc., and achieve the effect of high peptide yield

Inactive Publication Date: 2018-08-03
ZHEJIANG OCEAN UNIV
View PDF4 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in the processing of anglerfish, a large amount of fish skin is discarded as leftovers, which not only pollutes the environment, but also causes a waste of resources

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Preparation method and application of anglerfish bone antitumor decapeptide
  • Preparation method and application of anglerfish bone antitumor decapeptide
  • Preparation method and application of anglerfish bone antitumor decapeptide

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0020] The present invention will be described in detail below with reference to specific examples and experimental detection diagrams. The method for preparing an anglerfish bone anti-tumor decapeptide of the present invention uses anglerfish bone as a raw material, and the preparation method includes The following steps:

[0021] a) Crushed material: Use a high-speed tissue masher to mash the anglerfish bones to make powdered and micro-granular anglerfish bone powder;

[0022] b) Enzymatic hydrolysis: using neutral protease to enzymatically hydrolyze the anglerfish bone meal to make an anglerfish bone meal enzymatic hydrolysate;

[0023] c) Ultrafiltration: Add the enzymatic hydrolysate of Anglerfish bone meal to the ultrafiltration system, and use 10KD, 5KD, 3KD ultrafiltration membranes for ultrafiltration, and collect> 10KD, 10-5KD, 5-3KD and <3KD molecular weight enzymolysis solution, freeze-dried,

[0024] d) Pass the anglerfish enzymatic hydrolysate through G-25 eluate to obta...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a preparation method and an application of anglerfish bone antitumor decapeptide. The preparation method comprises the following steps: a) crushing: crushing anglerfish bones by a high-speed tissue crusher; b) performing enzymatic hydrolysis: performing enzymatic hydrolysis on above obtained anglerfish bone meal by a neutral protease to prepare an anglerfish bone meal enzymatic hydrolysate; and c) ultrafiltering: adding the anglerfish bone meal enzymatic hydrolysate into an ultrafiltration system, ultrafiltering the anglerfish bone meal enzymatic hydrolysate by a 10 KDultrafiltration membrane, a 5 KD ultrafiltration membrane and a 3 KD ultrafiltration membrane, collecting the enzymatic hydrolysate with the molecular weight respectively being more than 10 KD, 10-5 KD, 5-3 KD and less than 3 KD, and performing freeze drying.

Description

Technical field [0001] The invention relates to a preparation method and application of anglerfish bone anti-tumor decapeptide, in particular to a preparation method and application of anglerfish bone anti-tumor decapeptide with inhibitory effect on prostate cancer cells. Background technique [0002] Traditional treatment methods mainly include radical resection of prostate cancer, hormone therapy, chemotherapy, radiation therapy, etc., but the cancer is difficult to eradicate after treatment, and the recurrence rate is extremely high. Therefore, finding a new type of anti-tumor drug with high efficiency, low toxicity and strong specificity from the ocean has important research significance and clinical significance. [0003] During the processing of aquatic products, a large amount of scraps (including heads, skins, fins, tails, bones and residual fish flesh) will be produced, and its quality accounts for about 30% to 55% of the raw fish. Different parts The content of collagen ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12P21/06C07K7/06C07K1/36C07K1/34C07K1/20A61K38/08A61P35/00
CPCA61K38/00C07K7/06C12P21/06
Inventor 黄芳芳
Owner ZHEJIANG OCEAN UNIV