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Gene diagnosis composition and kit for predicting clopidogrel resistance of patient with stable angina

A technology for clopidogrel resistance and genetic diagnosis, applied in the biological field, can solve problems such as increased risk of bleeding, adverse cardiovascular events, and endangering the lives of patients

Inactive Publication Date: 2018-08-03
姜振东
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, once clopidogrel or aspirin resistance occurs, it will lead to the occurrence of adverse cardiovascular events after PCI treatment, greatly increase the risk of bleeding, and even endanger the lives of patients

Method used

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  • Gene diagnosis composition and kit for predicting clopidogrel resistance of patient with stable angina
  • Gene diagnosis composition and kit for predicting clopidogrel resistance of patient with stable angina
  • Gene diagnosis composition and kit for predicting clopidogrel resistance of patient with stable angina

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0059] Example 1: circRNAs composition and method for predicting clopidogrel resistance in patients with stable angina

[0060] 1. Experimental sample

[0061] Selected patients with coronary heart disease planned for PCI from Hunan Provincial People's Hospital from January 2016 to December 2017, and divided the patients with coronary heart disease into stable angina pectoris group according to the preoperative diagnosis (coronary angiography and electrocardiogram, etc.) ( SAP group), unstable angina group (UA group), ST-segment elevation myocardial infarction group (STEMI group) and non-ST-segment elevation myocardial infarction group (NSTEMI group). Before the operation, they were pre-treated with clopidogrel tablets (Polivix) with a loading dose of 300 mg. The maximum platelet aggregation rate (MPAR) induced by 5μM adenosine diphosphate was detected before and 12 hours after the drug. At the same time, the plasma before the drug was measured. The relative expression level of ta...

Embodiment 2

[0092] Example 2: circRNAs composition and method for predicting clopidogrel resistance in patients with unstable angina pectoris

[0093] 1. Experimental sample

[0094] The same as in Example 1.

[0095] 2. Experimental method

[0096] 1. Total RNA extraction and quality inspection

[0097] Follow the instructions of the TaKaRa RNAiso Blood kit. Take 250μL of plasma from each sample to extract the total RNA in the plasma sample. Use a spectrophotometer to determine the optical density D(260) and D(280) values ​​of total RNA, use D(260) value to calculate its concentration, and calculate D(260) / D(280) value to detect its purity, D(260) ) / D(280) value range of 1.8~2.1 is considered qualified.

[0098] 2. Real-time fluorescence quantitative PCR to detect target circRNAs

[0099] Operate according to the instructions of PrimeScript RT reagent Kit with gDNAEraser kit. The real-time fluorescent quantitative PCR primers for the target circRNAs and internal reference GAPDH were designed and ...

Embodiment 3

[0121] Example 3: circRNAs composition and method for predicting clopidogrel resistance in patients with ST-segment elevation myocardial infarction

[0122] 1. Experimental sample

[0123] The same as in Example 1.

[0124] 2. Experimental method

[0125] 1. Total RNA extraction and quality inspection

[0126] Follow the instructions of the TaKaRa RNAiso Blood kit. Take 250μL of plasma from each sample to extract the total RNA in the plasma sample. Use a spectrophotometer to determine the optical density D(260) and D(280) values ​​of total RNA, use D(260) value to calculate its concentration, and calculate D(260) / D(280) value to detect its purity, D(260) ) / D(280) value range of 1.8~2.1 is considered qualified.

[0127] 2. Real-time fluorescence quantitative PCR to detect target circRNAs

[0128] Operate according to the instructions of PrimeScript RT reagent Kit with gDNAEraser kit. The real-time fluorescent quantitative PCR primers for the target circRNAs and internal reference GAPDH ...

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Abstract

The invention discloses a gene diagnosis composition for predicting clopidogrel resistance of a patient with stable angina. The gene diagnosis composition discovers that circRNAs can be used for predicting whether coronary heart diseases in different types occurs the clopidogrel resistance or not and can evaluate the risk that the patients with the coronary heart diseases in different types occurthe clopidogrel resistance at early stage of a PCI surgery to be used as a reference basis whether the PCI surgery is performed or not or used for making an emergency plan of occurrence of the clopidogrel resistance after the PCI surgery as soon as possible.

Description

Technical field [0001] The invention belongs to the field of biology, and specifically relates to the prediction of antiplatelet drug resistance in patients with different types of coronary heart disease. Background technique [0002] Platelet activation and aggregation are involved in the entire development process of coronary heart disease. Aspirin and clopidogrel-based antiplatelet therapy is the cornerstone of drug treatment after coronary heart disease interventional therapy. The administration of aspirin and clopidogrel after PCI can delay vascular endothelialization, delay the process of restenosis and thrombosis, and significantly reduce the risk of adverse cardiovascular events, especially stent thrombosis. Clinical evidence shows that antiplatelet therapy can significantly reduce the occurrence of adverse cardiovascular events and reduce the risk of bleeding for patients with different types of coronary heart disease after PCI. [0003] In recent years, studies have foun...

Claims

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Application Information

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IPC IPC(8): C12Q1/6883C12N15/11
CPCC12Q1/6883C12Q2600/178
Inventor 姜振东周林飞董杏林
Owner 姜振东