Method for knocking out Endoglin gene by using CRISPR/Cas9 technology

A gene and technology technology, applied in the field of knocking out the Endoglin gene by using CRISPR/Cas9 technology, can solve the problem of weak tumor proliferation ability
CN108384784AInactive Publication Date: 2018-08-10GUANGXI MEDICAL UNIVERSITY
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
GUANGXI MEDICAL UNIVERSITY
Publication Date
2018-08-10
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses a method for knocking out an Endoglin gene by using a CRISPR / Cas9 technology, belonging to the technical fields of genetic engineering, immunology and oncology. The method comprises a CRISPR / Cas9 gRNA sequence for the Endoglin gene knockout; the sequence is shown in SEQ ID No. 1; the CRISPR / Cas9 gRNA sequence for the Endoglin gene knockout is designed in a first exon. The invention further studies the indispensable role of Endoglin in occurrence, development and metastasis processes of malignant tumor cells, and explores the effect of using a CRISPR / Cas9 gene editing technology to treat a mouse liver cancer model, so that a new ideal is provided for clinical tumor treatment.
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Description

technical field

[0001] The invention belongs to the technical fields of genetic engineering, immunology and oncology, and particularly relates to a method for knocking out Endoglin gene by using CRISPR / Cas9 technology and its application. Background technique

[0002] Tumor is an abnormal lesion formed by a certain cell in a local tissue losing its normal regulation of its growth at the gene level under the action of various carcinogenic factors, resulting in its clonal abnormal proliferation. In the late 20th century In the past 30 years, the incidence of cancer has been on the rise. According to the report of the World Health Organization (WHO), the number of new cancer cases in the world in 1990 was about 8.07 million, an increase of 37.4% from 5.17 million in 1975. The number of cancer deaths is about 6.2 million, and according to current trends, as the world population reaches 8 billion by 2020, there will be 20 million new cancer cases, of which the death toll will rea...

Claims

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