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Fluorescence lifetime imaging system and method for simultaneous measurement of photon arrival time and position

A time-of-arrival, synchronous measurement technology, used in measurement devices, fluorescence/phosphorescence, material analysis by optical means, etc., can solve the problems of limited spectral resolution channels, fixed wavelength intervals, and limited system applications, and achieve high data measurement. The effect of precision, high spectral resolution, and multi-research value

Active Publication Date: 2021-01-19
NANCHANG UNIV
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Problems solved by technology

This leads to limited spectral resolution channels of the system, fixed wavelength intervals, and the low imaging rate of the TCSPC technology itself, which limits the application of this system

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  • Fluorescence lifetime imaging system and method for simultaneous measurement of photon arrival time and position
  • Fluorescence lifetime imaging system and method for simultaneous measurement of photon arrival time and position
  • Fluorescence lifetime imaging system and method for simultaneous measurement of photon arrival time and position

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Embodiment Construction

[0041] Below in conjunction with accompanying drawing and embodiment the present invention is further described:

[0042] see Figure 1-3 .

[0043] The invention discloses a fluorescence lifetime imaging system for synchronous measurement of photon arrival time and position, such as figure 1 As shown, it includes a scanning translation stage 1, a sample to be imaged 10, an excitation light source 2, a focusing lens 3, a polychromator 4, an MCP position-sensitive anode detector 5, a time signal extraction module 6, a charge-sensitive preamplifier 7, and a photon arrival Time and position synchronization measurement circuit 8 and image reconstruction module 9 .

[0044] The scanning translation stage 1 is used to place the sample 10 to be imaged, and the excitation light source 2 is used to irradiate the sample 10 to be imaged, and it is characterized in that: a focusing lens 3, a polychromator 4, and an MCP position-sensitive anode detector 5 are arranged in sequence In the...

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Abstract

The invention relates to a fluorescence lifetime imaging system and method for synchronous measurement of photon arrival time and position. The system includes a scanning translation stage, a to-be-imaged sample, an excitation light source, a focusing lens, a polychromator, and an MCP position sensitive anode detector. The scanning translation stage is used for placing the to-be-imaged sample. Theexcitation light source is used for irradiating the to-be-imaged sample. The focusing lens, the polychromator, and the MCP position sensitive anode detector are arranged in the excited light propagation direction of the to-be-imaged sample in order. The outside of the MCP position sensitive anode detector is connected to a time signal extraction module. The position sensitive anode multiplexed output of the MCP position sensitive anode detector is connected to a charge sensitive preamplifier, a photon arrival time and position synchronous measurement circuit, and an image reconstruction module in order. The time signal extraction module is connected to the photon arrival time and position synchronous measuring circuit. And the scanning translation stage is connected to the image reconstruction module. The system and method provided by the invention can achieve finer spectral resolution and can acquire more dimensional information.

Description

technical field [0001] The invention relates to the technical field of fluorescence lifetime imaging, in particular to a fluorescence lifetime imaging system for synchronous measurement of photon arrival time and position, and also relates to an imaging method of the system. Background technique [0002] Fluorescence lifetime imaging (FLIM) is a new type of fluorescence imaging technology that uses the fluorescence lifetime measured at a pixel as the corresponding gray value of the pixel. , fluorescent dye bleaching, uneven distribution of fluorescent dyes and other interference factors of fluorescence intensity. Therefore, fluorescence lifetime imaging technology can provide clearer contrast to biological tissues in different states or different types of biological tissues, and can quantitatively measure fluorophores and their microenvironmental parameters. Fluorescence lifetime imaging technology has been successfully applied in the fields of cell biology, analytical chem...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/64G01N21/01
CPCG01N21/01G01N21/6402
Inventor 鄢秋荣王慧袁成龙李冰
Owner NANCHANG UNIV
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