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A kind of quality detection method of Huoshan Dendrobium

A quality detection method, the technology of Dendrobium Huoshanense, applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems that cannot fully reflect the overall curative effect, achieve pertinence and applicability of interpretation, realize correlation analysis, and accurate quality evaluation Effect

Active Publication Date: 2021-03-16
北京蓝标一成科技有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In traditional Chinese medicine under the guidance of traditional Chinese medicine theory, any active ingredient cannot fully reflect the overall curative effect of traditional Chinese medicine.

Method used

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  • A kind of quality detection method of Huoshan Dendrobium
  • A kind of quality detection method of Huoshan Dendrobium
  • A kind of quality detection method of Huoshan Dendrobium

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0052] The generation sequencing of embodiment 1 Huoshan Dendrobium

[0053] First-generation sequencing primer sequences:

[0054] ITS-26SE: 5'GAATTCCCCGGTTCGCTCGCCGTTAC 3';

[0055] ITS-17SE: 5' ACGAATTCATGGTCCGGTGAAGTGTTCG 3'.

[0056] The amplification and sequencing parameters are: denaturation at 98°C for 2 minutes and then PCR cycle, the PCR cycle parameters are 98°C for 20s; 52°C for 30s; 68°C for 1min, 38 cycles, 68°C for 7min, set 4°C after the amplification, and perform Generation Molecular Sequencing.

[0057] Through the first-generation sequencing, the species of Dendrobium to be tested was identified as Dendrobium Huoshanense.

Embodiment 2

[0058] The extraction method of embodiment 2 Huoshan Dendrobium

[0059] Take the dried sample of Dendrobium Huoshanense, pulverize it with a pulverizer, pass through a pharmacopoeia sieve (aperture 0.335mm), accurately weigh 1.000g of Dendrobium powder (the weighing error cannot exceed 0.2%), place it in a 100ml Erlenmeyer flask, and add 50mL of 75% Methanol (V water: V methanol = 25:75), after ultrasonication for 30 minutes at room temperature, it was taken out, filtered, the filtrate was concentrated to dryness by rotary evaporation, dissolved in 75% methanol solvent (V water: V methanol = 25:75), and finally transferred to Dilute to volume in a 10ml volumetric flask, shake well, and filter with a 0.45 μm microporous membrane to obtain a sample solution of Dendrobium Huoshanense.

Embodiment 3

[0060] The chromatographic detection method of the Dendrobium Huoshanense extract of embodiment 3

[0061] ①Preparation of reference solution

[0062] Accurately weigh 4.10 mg of Schaftoside and 4.08 mg of naringenin, place them in 10 ml volumetric flasks, add 75% (V / V) methanol to dissolve and dilute, shake well, and use them as stock solutions. Refrigerate at 4°C for later use.

[0063] Then accurately draw a certain amount of reference substance stock solution and dilute with 75% methanol to accurately prepare a mixed reference substance solution of schaffertoside and naringenin. Through different dilution ratios, 7 concentration points of the prepared components were diluted. into a high performance liquid chromatograph.

[0064] ②Extraction and processing method of samples for determination of small molecular components of Dendrobium Huoshanense:

[0065] Take 1.00g of the powder of this product (passed through a No. 3 sieve), accurately weigh it, put it in a 100ml vo...

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Abstract

The invention discloses a quality detection method of Dendrobium huoshanense. The quality detection method comprises: 1) sequencing by using ITS-26SE and ITS-17SE as primers to identify the variety ofa to-be-detected Dendrobium herb; 2) carrying out chromatographic detection on n samples to obtain detection data using a chemical small molecule component schaftoside and / or naringenin as a reference component; 3) respectively carrying out fingerprint detection on the samples to obtain the fingerprint peak area value of the full chemical components of Dendrobium huoshanense; and 4) establishingan analysis model by using the content value of the schaftoside and / or naringenin in the chromatographic data as the response variable and using the peak area value of other components in the fingerprint as the independent variable, screening the variable through a Lasso method, and establishing the characteristic fingerprint model related to the chemical small molecule component. According to thepresent invention, through the first-generation sequencing and the characteristic fingerprinting, the quality of Dendrobium huoshanense is precisely identified and controlled.

Description

technical field [0001] The invention belongs to the field of detection and analysis of traditional Chinese medicine components, and in particular relates to a quality detection method of Dendrobium huoshanense. Background technique [0002] Dendrobium huoshanense (Dendrobium huoshanense C.Z.Tang&S.J.Cheng) is also known as Huoshanense. Stem erect, fleshy, densely planted, 3cm-9cm long, tapering from base upwards, 0.3cm-1.0cm in diameter above base, unbranched, with 3-7 nodes, internodes 0.3cm-0.8cm long, Pale yellowish green, sometimes with lavender spots, light yellow when dry. Leaf bases with clasping sheaths, sheaths membranous, persistent. 1-3 racemes, issued from the upper part of the old stems of deciduous leaves, with 1-2 flowers; inflorescence peduncle 0.2cm-0.3cm long, with 1-2 sheaths at the base; sheaths are papery, egg-shaped Needle-shaped, 0.3cm-0.4cm long, with sharp apex; bracts light white with maroon, ovate, 0.3cm-0.4cm long, with sharp apex; pedicels and...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/06G01N30/74G01N30/86
CPCG01N30/06G01N30/74G01N30/8686
Inventor 赵田
Owner 北京蓝标一成科技有限公司
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