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Ultra-early detection kit for serum miRNAs in patients with alcoholic femoral head necrosis

A femoral head necrosis, alcohol-based technology, applied in the direction of DNA / RNA fragments, recombinant DNA technology, microbial measurement / inspection, etc., to achieve good clinical application prospects and good accuracy

Active Publication Date: 2022-03-15
陈镇秋 +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, there is still a gap with current technology in trying to provide sufficient diagnostic information for early diagnosis in patients with AIONFH

Method used

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  • Ultra-early detection kit for serum miRNAs in patients with alcoholic femoral head necrosis
  • Ultra-early detection kit for serum miRNAs in patients with alcoholic femoral head necrosis
  • Ultra-early detection kit for serum miRNAs in patients with alcoholic femoral head necrosis

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] (1) RT-PCR primer synthesis:

[0035] Synthesize miR-127-3p, miR-1, miR-628-3p, miR-432-5p, miR-483-3p, miR-483-5p, miR-885-5p, miR-423-5p according to the following table ( The sequences of RT-PCR detection primers are shown in Table 1.

[0036] Table 1 is the sequence of RT-PCR detection primer GSP and primer R of each miR

[0037]

[0038]

[0039] (2) Detection of miRs expression level

[0040] 1) Synthesis of cDNA template by reverse transcription

[0041] RNA was extracted from the serum or plasma of AIONFH patients and non-AIONFH patients, and reverse-transcribed into cDNA templates. The reaction system for cDNA synthesis by reverse transcription is as follows:

[0042]

[0043] The prepared cDNA template, nuclease free water and SYBR TM Put the Green master mix on ice to dissolve for 15-20 minutes. SYBR TM Green master mix should be dissolved in the dark, and should be inverted before use, and other reagents should be shaken to mix. All reagents...

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Abstract

The invention discloses a serum miRNAs detection kit for patients with early alcoholic femoral head necrosis. Primer sequences for 3p, miR‑483‑5p, miR‑885‑5p. The 7 miRs of the present invention have good accuracy in diagnosing ultra-early alcoholic femoral head necrosis; the sensitivity and specificity of some miRs can reach 100.00% and 100.00%, and have good clinical application prospects.

Description

technical field [0001] The invention relates to the field of biological detection, in particular to an ultra-early detection kit for serum miRNAs in patients with alcoholic femoral head necrosis. Background technique [0002] Alcoholic necrosis of the femoral head (AIONFH) is one of the pathological processes of apoptosis in bone tissue caused by drinking alcoholic beverages proposed by the World Health Organization (WHO). AIONFH, if left untreated, can lead to collapse of the femoral head, and about 80 percent of AIONFH patients require hip replacement, a devastating and irreversible disease. Although the pathogenesis of AIONFH is based on various theories, such as the theory of intravascular coagulation and the concept of intravascular fat embolism, it is still unclear. For now, there is no gold standard for early diagnosis and evaluation of AIONFH so far. Only histological examination and magnetic resonance imaging (MRI) can be used as the earliest basis for diagnosis, ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6883C12N15/11
CPCC12Q1/6883C12Q2600/158C12Q2600/178
Inventor 陈镇秋洪郭驹魏秋实何伟韩晓蕊
Owner 陈镇秋