Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Delivery molecule and nanoparticle, preparation method and application

A nanoparticle and molecular technology, applied in the field of pharmaceutical preparations, can solve the problems of delivery system toxicity and delivery efficiency, and achieve high delivery efficiency and the effect of inhibiting influenza virus

Inactive Publication Date: 2018-08-21
QINGDAO AGRI UNIV
View PDF2 Cites 6 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Therefore, the technical problem to be solved by the present invention is to overcome the problems of toxicity and low delivery efficiency in the delivery system in the prior art, so as to provide a non-toxic and high delivery efficiency delivery molecule and nanoparticle, preparation method and use

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Delivery molecule and nanoparticle, preparation method and application
  • Delivery molecule and nanoparticle, preparation method and application
  • Delivery molecule and nanoparticle, preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Example 1 Delivery Molecule T9(dR)

[0036] A kind of delivery molecule T9(dR) provided in this embodiment, its cell-penetrating peptide selects the transpotan (complex of neuropeptide N-terminus and wasptoxin cell membrane binding region), and the C-terminus of the transpotan is linked with 9-polyarginine (dR9) forms a composite peptide, and its amino acid sequence is as follows: GWTLNSAGYLLGKINLKALAALAKKILdRdRdRdRdRdRdRdRdRdR, and its amino acid sequence is shown in SEQ ID NO:1. The above composite peptide was named as T9(dR). The above compound peptides were prepared by GenScript.

Embodiment 2

[0037] Example 2 Delivery Molecule T7(dR)

[0038] In the delivery molecule T7(dR) provided in this example, its cell-penetrating peptide selects the transpotan (a complex of the neuropeptide N-terminus and the wasptoxin cell membrane-binding region), and the N-terminus of the transpotan is linked with 7-polyarginine (dR7) forms a composite peptide, and its amino acid sequence is as follows: dRdRdRdRdRdRdRdRGWTLNSAGYLLGKINLKALAALAKKIL, and its amino acid sequence is shown in SEQ ID NO:2. The above composite peptide was named as T7(dR). The above compound peptides were prepared by GenScript.

Embodiment 3

[0039] Example 3 Delivery Molecule T10(dR)

[0040] A kind of delivery molecule T10(dR) provided in this example, its cell-penetrating peptide selects transpotan (a complex of neuropeptide N-terminal and wasptoxin cell membrane-binding region), and the C-terminus of the transpotan is linked with 10-polyarginine (dR10) forms a composite peptide, and its amino acid sequence is as follows: GWTLNSAGYLLGKINLKALAALAKKILdRdRdRdRdRdRdRdRdRdR. The above compound peptide was named as T10(dR). The above compound peptides were prepared by GenScript.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
diameteraaaaaaaaaa
Login to View More

Abstract

The invention provides a delivery molecule and a nanoparticle, a preparation method and application. The delivery molecule comprises cell penetrating peptide, poly-L-arginine is linked to the C or N end of the cell penetrating peptide, the delivery molecule can combine with siRNA and other nucleotides, protein and DNA to form a nanoparticle with a diameter of 600nm, the delivery molecule can deliver siRNA into cells, and release siRNA to play a therapeutic role. The invention indicates that the delivery molecule can deliver transferred substances into cells or animal bodies both on the cell and animal level and release the transferred substances to play a part, and the delivery molecule is non-toxic and has high delivery efficiency.

Description

technical field [0001] The invention belongs to the technical field of pharmaceutical preparations, and specifically relates to a delivery molecule and nanoparticle, a preparation method and an application. Background technique [0002] The delivery of polynucleotides and other drugs that are membrane impermeable to living cells is limited due to the complex membrane system of cells. Commonly used antisense, RNAi and gene therapy drugs are relatively large hydrophilic polymers and often carry a high negative charge, and the two physical characteristics of hydrophilicity and high negative charge severely limit the crossover of these drugs. The cell membrane diffuses directly. Thus, a major obstacle to the delivery of polynucleotides, as well as other drugs, is delivery across the cell membrane to the cytoplasm or nucleus. [0003] Currently, numerous transfection reagents have been developed that achieve moderately efficient delivery of polynucleotides into cells in vitro, ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/00A61K9/14A61K47/64A61K31/713A61P31/16
CPCA61K9/14A61K31/713A61K47/64A61P31/16C07K14/00
Inventor 李军伟刘光辉刘玉萍单虎
Owner QINGDAO AGRI UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com