High-throughput continuous flow analysis method for total plant alkaloid in tobacco gene editing material
A gene editing and flow analysis technology, applied in the field of tobacco chemistry research, can solve the problems of affecting the reliability of test results, not being simple enough, affecting the efficiency of detection work, etc. Effect
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Embodiment 1
[0030] Embodiment 1: Determination of total plant alkaloids in flue-cured tobacco sample A
[0031] 1) Sample pretreatment: first dry the shredded tobacco, and use a high-speed cyclone mill to break the cell wall of tobacco and tobacco products, then pass through a 60-mesh sieve, and seal and store as a sample. Test the water content of the sample according to the YC / T296-2009 standard, and then handle it as follows:
[0032] A. Accurately weigh 0.25g of sample into a 25mL improved sample tube, remove the sample tray from the automatic sampler of the continuous flow analyzer, and fill the sample tube with the weighed sample in order to inject into the continuous flow analyzer 100 sampling tube positions in the tray;
[0033] B. Use a 25mL pipette gun to add 25mL of deionized water to each sample tube, and then insert the inner casing of the filter sieve plate with a pore size of 0.45μm at the bottom in order to prevent the solution from splashing out during the vibration extr...
Embodiment 2
[0037] Embodiment 2: Determination of total plant alkaloids in finished cigarette sample B
[0038] The process in Example 1 was repeated to measure the total phytoalkaloids in the finished cigarette sample B, and the total phytoalkaloid content in the sample was measured to be 4.25%.
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