A bacterial strain for preventing and controlling plant nematodes and its application

A plant line and strain technology, applied in the field of microbiology, can solve the problems that the research on plant nematode diseases has not been reported, and achieve good effects on the control of plant nematode activity, ecological environment and human health, and reduce the degree of pollution

Active Publication Date: 2020-06-23
HUNAN AGRICULTURAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The currently reported biocontrol bacteria with nematicidal potential mainly include a variety of fungi and different types of biocontrol bacteria, but the research on the use of Marseilles strains to control plant nematode diseases has not been reported

Method used

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  • A bacterial strain for preventing and controlling plant nematodes and its application
  • A bacterial strain for preventing and controlling plant nematodes and its application
  • A bacterial strain for preventing and controlling plant nematodes and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Embodiment 1: Isolation and identification of Marseilles strain Y149

[0030] (1) Separation and purification

[0031] Beef extract peptone agar medium: 3g beef extract, 10g peptone, 10g sodium chloride, 15g agar, 1000mL deionized water, pH7.4, sterilized at 121°C for 30min.

[0032] The present inventor collected plant rhizosphere soil samples from Changsha City, Hunan Province, and after natural air drying, weighed 10 g of the samples and dissolved them in 90 ml of sterile water to make a soil suspension. Then use 10-fold gradient dilution with sterile water, draw 10 -3 and 10 -4 100 μl of the soil suspension was evenly spread on the beef extract peptone medium plate. Put the above plate into the incubator at 30°C for 36h-48h, pick different colonies according to the shape, color and size of the colony, and purify them on the new beef extract peptone medium plate until the pure colonies are obtained and numbered for preservation .

[0033] (2) Morphological and p...

Embodiment 2

[0046] Embodiment 2: the preparation of microbial bacterial agent

[0047]Beef extract peptone broth (NB): 3g beef extract, 10g peptone, 10g sodium chloride, 1000mL deionized water, pH 7.4, sterilized at 121°C for 30min.

[0048] (1) Cultivation of slant seeds: Aseptically streak the thallus of Massilia sp. Y149 onto the slant of beef extract peptone agar medium, culture at 30°C for 36 hours to obtain slant seeds;

[0049] (2) Liquid seed culture: Pick an activated single colony from the slope and inoculate it in NB medium for culture, 200r / min, 30°C, culture time is 24h, to obtain the seed culture solution;

[0050] (3) Fermentation culture: insert the seed culture liquid into the NB culture medium according to the inoculation amount of 5%, at 30°C, the rotating speed is 200r / min, and the fermentation time is 72h to obtain a fermented liquid with OD600=4, and the number of viable bacteria is 3.5× 10 8 cfu / ml;

[0051] (4) Preparation of microbial agent: collect the ferment...

Embodiment 3

[0052] Example 3: Detection of the activity of lethal plant nematodes by Marseilles strain Y149

[0053] Potato dextrose agar medium (PDA): 200g of potatoes, 20g of glucose, 15g-20g of agar, 1000mL of water.

[0054] (1) Preparation of fermentation supernatant: Marseilles strain Y149 was fermented and cultured for 72 hours according to the method in Example 2 to obtain a fermentation system suspension with OD600=4, centrifuged at 10000r / min for 10min, and the supernatant was collected for later use.

[0055] (2) Preparation of nematode suspension: D. destructor potato used in this example was preserved by the Plant Nematode Laboratory of Hunan Agricultural University. Before culturing, inoculate Fusarium half naked on a PDA plate, and incubate at 25°C for 7-10 days. After the mycelia cover the entire plate, insert 100 μL of D. destructor suspension under aseptic conditions, and incubate at 25°C for 7-10 days. After the half-naked Fusarium hyphae disappeared, the nematodes cov...

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Abstract

The invention relates to a bacterial strain for preventing and controlling plant nematodes and its application. The bacterial strain Y149 provided by the present invention belongs to the genus Massilia sp., and has been deposited in the China Center for Type Culture Collection on November 27, 2017. The address of the preservation unit is Wuhan University, Wuhan, China, and the preservation number is CCTCC NO: M 2017745. The present invention also provides the microbial bacterial agent containing the bacterial strain Y149 and its preparation method, and applies the above-mentioned bacterial strain Y149 or the microbial bacterial agent to the preparation of products for preventing and controlling plant nematodes. The bacterial strain Y149 or microbial bacterial agent of the present invention has a good control effect on plant nematodes, and is low in cost and friendly to the environment.

Description

technical field [0001] The invention relates to the field of microorganisms, in particular to a bacterial strain for preventing and controlling plant nematodes and its application. Background technique [0002] Nematodes (nematodes) belong to the class Nematoda of the phylum Nematoda, and can be divided into free-living nematodes, animal-parasitic nematodes and plant-parasitic nematodes according to their life cycle and living environment. Among them, plant-parasitic nematodes have become a worldwide soil-borne pathogen that threatens agricultural production due to their wide host range, strong pathogenicity, fast self-reproduction and easy transmission. At present, plant parasitic nematodes are the main factors restricting the sustainable development of agriculture in our country. [0003] At present, in agricultural production, the method of controlling plant parasitic nematodes mainly relies on traditional chemical control, but chemical agents are usually highly toxic, a...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A01N63/20A01P5/00C05F11/08C12R1/01
CPCA01N63/00A01N63/10C05F11/08C12N1/20C12N1/205C12R2001/01
Inventor 叶姗丁中肖宓周建宇姚思敏袁涛曾润娥
Owner HUNAN AGRICULTURAL UNIV
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