Application of Hydroquinolins in Enhancing the Sensitivity of Bacteria to Colistin
A polymyxin and compound technology, applied in the new application field of compounds, can solve problems such as resensitization
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Embodiment 1
[0046] The influence of embodiment 1 hydroquinolins compound on bacterial growth
[0047] 1) Bacterial culture: 4 strains of MCR-1 positive drug-resistant bacteria and Escherichia coli standard strain ATCC25922 were selected as experiments. After the solid LB plate was revived, single colonies were selected and cultured overnight with MH medium to enrich the bacteria.
[0048] 2) Bacterial suspension preparation: Prepare a 0.5 McFarland turbidity bacterial suspension with physiological saline, and dilute it with MH culture medium 1:100 to make the bacterial content 1.5×10 6 cfu / ml, inoculate 50 μl per well in a 96-well plate.
[0049] 3) Add 50 μl of various hydroquinolin compounds (as shown in Table 1) with a concentration of 100 μM in a 96-well plate, and inoculate 4 strains of MCR-1-positive drug-resistant bacteria and ATCC25922 into 96 wells, and each strain of bacteria Inoculate 50 μl of bacterial solution, so that the total volume of each well is 100 μl, and the concen...
Embodiment 2
[0055] Example 2 High Concentration Hydroquinolins Enhance Bacteria's Effect on Colistin Sensitivity
[0056] 1) Bacterial culture: 4 strains of MCR-1-positive drug-resistant bacteria were selected as experimental strains, and Escherichia coli standard strain ATCC25922 was used as quality control bacteria. After the solid LB plate was revived, single colonies were selected and cultured overnight with MH medium to enrich the bacteria.
[0057] 2) Bacterial suspension preparation: Prepare a 0.5 McFarland turbidity bacterial suspension with physiological saline, and dilute it with MH culture medium 1:100 to make the bacterial content 1.5×10 6 cfu / ml, inoculate 50 μl per well in a 96-well plate.
[0058]3) Add 25 μl of polymyxin with a concentration of 64 mg / ml to the 96-well plate. Then continue to add 25 μl of various hydroquinolins (see Table 1) at a concentration of 200 μM in the 96-well plate, and inoculate 4 strains of MCR-1 positive drug-resistant bacteria and ATCC25922 i...
Embodiment 3
[0061] Example 3 Low-concentration hydroquinolins enhance the effect of bacteria on polymyxin sensitivity
[0062] 1) Bacterial culture: select 4 strains of MCR-1-positive drug-resistant bacteria and Escherichia coli standard strain ATCC25922 (without mcr-1 gene) as the experimental strains, and after recovering with solid LB plates, select single colonies respectively, and use MH medium overnight Cultivate, enrich.
[0063] 2) Bacterial suspension preparation: Prepare a 0.5 McFarland turbidity bacterial suspension with physiological saline, and dilute it with MH culture medium 1:100 to make the bacterial content 1.5×10 6 cfu / ml, inoculate 50 μl per well in a 96-well plate.
[0064] 3) Dilute each hydroquinolin compound of the present invention and polymyxin (6 dilutions, 2 times of MIC value, 1 time, 1 / 2, 1 / 4, 1 / 8, 1 / 16 concentration respectively) dilution). The horizontal direction is the direction of the descending gradient of the antibiotic, and the vertical direction i...
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