Microbial agent capable of preventing and controlling apple tree root rot disease as well as preparation method thereof

Abstract

The invention discloses a microbial agent capable of preventing and controlling an apple tree root rot disease as well as a preparation method thereof. The microbial agent capable of preventing and controlling the apple tree root rot disease is prepared from the following raw materials in parts by weight: 20 to 30 parts of trichoderma harzianum, 10 to 20 parts of bacillus natto, 15 to 25 parts ofbacillus subtilis, 20 to 30 parts of hansenula anomala, 5 to 15 parts of azotobacter chroococcum and 5 to 10 parts of porous magnetic chitosan microsphere. The microbial agent capable of preventing and controlling apple tree root rot disease completely avoids the problem of chemical medicine resistance caused by use of chemical agents, so that the microbial agent is favorable for pollution-free production of apples. A biological flora, which is complex, stable in structure and wide in function and has various bacterial microflora, is formed by depending on the mutual synergic effect and proliferation relationship and has a good prevention and control effect on the apple tree root rot disease.

Description

technical field

[0001] The invention belongs to the field of biology, and in particular relates to a microbial agent capable of preventing and controlling apple tree root rot and a preparation method thereof. Background technique

[0002] Apples belong to the family Rosaceae and are deciduous trees with strong polarity. They usually grow vigorously, with tall crowns, and the tree height can reach 15 meters. Under cultivation conditions, the tree is generally about 3-5 meters high. Trunk grayish brown, old bark with irregular longitudinal fissures or peeling off, branchlets smooth. According to measurements, every hectogram of apples contains 6.5-11.2 grams of fructose, 2.5-3.5 grams of glucose, 1.0-5.2 grams of sucrose, and also contains trace elements zinc, calcium, phosphorus, iron, potassium and vitamin B1, vitamin B2, vitamin C and carrots. Su and so on. Apple likes light and slightly acidic to neutral soil. It is most suitable for sandy soil with deep soil layer, rich...

Examples

Embodiment 1

[0033] A microbial bacterial agent capable of preventing and controlling apple tree root rot is prepared from the following raw materials in parts by weight: 20 parts of Trichoderma harzianum, 10 parts of Bacillus natto, 25 parts of Bacillus subtilis, and 30 parts of Hansenula anomaly , 5 parts of Azotobacter torus, 10 parts of porous magnetic chitosan microspheres.

[0034] Described, porous magnetic chitosan microsphere is made by following steps:

[0035] 1) Mix ammonia water, nano ferric oxide, carboxymethyl chitosan and Tween-20 evenly, seal and soak for 2 hours, heat in a water bath at 55°C and stir until carboxymethyl chitosan is completely dissolved, and stand still until bubbles are completely Escape, get carboxymethyl chitosan phase;

[0036] 2) Add Span-80 and liquid paraffin into the flask, heat in a water bath at 50°C, stir until evenly mixed, and use it as the paraffin phase;

[0037] 3) Add the carboxymethyl chitosan phase to the paraffin phase dropwise while ...

Embodiment 2

[0051] A microbial bacterial agent capable of preventing and controlling apple tree root rot is prepared from the following raw materials in parts by weight: 25 parts of Trichoderma harzianum, 15 parts of Bacillus natto, 20 parts of Bacillus subtilis, and 25 parts of Hansenula anomaly , 10 parts of Azotobacter torus, and 8 parts of porous magnetic chitosan microspheres.

[0052] Described, porous magnetic chitosan microsphere is made by following steps:

[0053] 1) Mix ammonia water, nano ferric oxide, carboxymethyl chitosan and Tween-20 evenly, seal and soak for 3 hours, heat in a water bath at 53°C and stir until carboxymethyl chitosan is completely dissolved, and stand still until the bubbles are completely Escape, get carboxymethyl chitosan phase;

[0054] 2) Add Span-80 and liquid paraffin into the flask, heat in a water bath at 55°C, stir until evenly mixed, and use it as the paraffin phase;

[0055] 3) Under the condition of heating in a water bath at 55°C, add the ca...

Embodiment 3

[0069] A microbial bacterial agent capable of preventing and controlling apple tree root rot is prepared from the following raw materials in parts by weight: 30 parts of Trichoderma harzianum, 20 parts of Bacillus natto, 15 parts of Bacillus subtilis, and 20 parts of Hansenula anomaly , 15 parts of Azotobacter torus, and 5 parts of porous magnetic chitosan microspheres.

[0070] Described, porous magnetic chitosan microsphere is made by following steps:

[0071] 1) Mix ammonia water, nano ferric oxide, carboxymethyl chitosan and Tween-20 evenly, seal and soak for 4 hours, heat in a water bath at 50°C and stir until carboxymethyl chitosan is completely dissolved, and stand still until the bubbles are completely Escape, get carboxymethyl chitosan phase;

[0072] 2) Add Span-80 and liquid paraffin into the flask, heat in a water bath at 60°C, stir until evenly mixed, and use it as the paraffin phase;

[0073] 3) Add the carboxymethyl chitosan phase to the paraffin phase dropwis...

technical field

[0001] The invention belongs to the field of biology, and in particular relates to a microbial agent capable of preventing and controlling apple tree root rot and a preparation method thereof. Background technique

[0002] Apples belong to the family Rosaceae and are deciduous trees with strong polarity. They usually grow vigorously, with tall crowns, and the tree height can reach 15 meters. Under cultivation conditions, the tree is generally about 3-5 meters high. Trunk grayish brown, old bark with irregular longitudinal fissures or peeling off, branchlets smooth. According to measurements, every hectogram of apples contains 6.5-11.2 grams of fructose, 2.5-3.5 grams of glucose, 1.0-5.2 grams of sucrose, and also contains trace elements zinc, calcium, phosphorus, iron, potassium and vitamin B1, vitamin B2, vitamin C and carrots. Su and so on. Apple likes light and slightly acidic to neutral soil. It is most suitable for sandy soil with deep soil layer, rich...

Examples

Embodiment 1

[0033] A microbial bacterial agent capable of preventing and controlling apple tree root rot is prepared from the following raw materials in parts by weight: 20 parts of Trichoderma harzianum, 10 parts of Bacillus natto, 25 parts of Bacillus subtilis, and 30 parts of Hansenula anomaly , 5 parts of Azotobacter torus, 10 parts of porous magnetic chitosan microspheres.

[0034] Described, porous magnetic chitosan microsphere is made by following steps:

[0035] 1) Mix ammonia water, nano ferric oxide, carboxymethyl chitosan and Tween-20 evenly, seal and soak for 2 hours, heat in a water bath at 55°C and stir until carboxymethyl chitosan is completely dissolved, and stand still until bubbles are completely Escape, get carboxymethyl chitosan phase;

[0036] 2) Add Span-80 and liquid paraffin into the flask, heat in a water bath at 50°C, stir until evenly mixed, and use it as the paraffin phase;

[0037] 3) Add the carboxymethyl chitosan phase to the paraffin phase dropwise while ...

Embodiment 2

[0051] A microbial bacterial agent capable of preventing and controlling apple tree root rot is prepared from the following raw materials in parts by weight: 25 parts of Trichoderma harzianum, 15 parts of Bacillus natto, 20 parts of Bacillus subtilis, and 25 parts of Hansenula anomaly , 10 parts of Azotobacter torus, and 8 parts of porous magnetic chitosan microspheres.

[0052] Described, porous magnetic chitosan microsphere is made by following steps:

[0053] 1) Mix ammonia water, nano ferric oxide, carboxymethyl chitosan and Tween-20 evenly, seal and soak for 3 hours, heat in a water bath at 53°C and stir until carboxymethyl chitosan is completely dissolved, and stand still until the bubbles are completely Escape, get carboxymethyl chitosan phase;

[0054] 2) Add Span-80 and liquid paraffin into the flask, heat in a water bath at 55°C, stir until evenly mixed, and use it as the paraffin phase;

[0055] 3) Under the condition of heating in a water bath at 55°C, add the ca...

Embodiment 3

[0069] A microbial bacterial agent capable of preventing and controlling apple tree root rot is prepared from the following raw materials in parts by weight: 30 parts of Trichoderma harzianum, 20 parts of Bacillus natto, 15 parts of Bacillus subtilis, and 20 parts of Hansenula anomaly , 15 parts of Azotobacter torus, and 5 parts of porous magnetic chitosan microspheres.

[0070] Described, porous magnetic chitosan microsphere is made by following steps:

[0071] 1) Mix ammonia water, nano ferric oxide, carboxymethyl chitosan and Tween-20 evenly, seal and soak for 4 hours, heat in a water bath at 50°C and stir until carboxymethyl chitosan is completely dissolved, and stand still until the bubbles are completely Escape, get carboxymethyl chitosan phase;

[0072] 2) Add Span-80 and liquid paraffin into the flask, heat in a water bath at 60°C, stir until evenly mixed, and use it as the paraffin phase;

[0073] 3) Add the carboxymethyl chitosan phase to the paraffin phase dropwis...