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Application of ZFPM2-AS1 to preparation of pancreas-related disease diagnostic kit

A technology of ZFPM2-AS1, 1. ZFPM2-AS1, applied in the field of medical biological detection, can solve the problem of less ZFPM2-AS1, and achieve the effect of less pain and harm, high clinical reference value, high reliability and high sensitivity

Active Publication Date: 2018-09-11
SHANGHAI CHANGHAI HOSPITAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are very few studies on ZFPM2-AS1
At present, there is no literature report on the application of ZFPM2-AS1 in the diagnosis of pancreas-related diseases, especially pancreatic cancer

Method used

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  • Application of ZFPM2-AS1 to preparation of pancreas-related disease diagnostic kit
  • Application of ZFPM2-AS1 to preparation of pancreas-related disease diagnostic kit
  • Application of ZFPM2-AS1 to preparation of pancreas-related disease diagnostic kit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] Example 1 Making a standard map of the expression level of ZFPM2-AS1 in pancreatic cancer, chronic pancreatitis and normal human plasma

[0047] 1. Plasma collection

[0048] 48 outpatients or inpatients in Shanghai Changhai Hospital from January 2017 to December 2017, including 33 cases of pancreatic cancer and 15 cases of chronic pancreatitis. The healthy control group included 16 cases, all of whom had normal physical examination in the hospital during the same period.

[0049] 2. Experimental method

[0050] 1. Plasma sample collection

[0051] 1) Plasma sample collection

[0052] Several blood samples were taken from patients with pancreatic cancer, patients with chronic pancreatitis, and normal people, and each blood sample was centrifuged at 3000r / min for 10min at room temperature, and it can be seen that it was divided into two layers. Absorb the upper layer of transparent light yellow liquid, that is, plasma, and divide it into 1.5ml EP tubes, and store the...

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Abstract

The invention relates to the technical field of medical biological detection, provides novel application of ZFPM2-AS1, and particularly relates to application of the ZFPM2-AS1 to preparation of a pancreas-related disease diagnostic kit. The invention further provides a kit for performing pancreas-related disease diagnosis by means of quantitative PCR (Polymerase Chain Reaction) detection specificto the ZFPM2-AS1 and a detection method. The kit and the detection method disclosed by the invention have the advantages of easiness, convenience, reliability, short period, high specificity and easiness in clinical popularization.

Description

technical field [0001] The invention relates to the technical field of medical biological detection, and relates to the application of lncRNA in the diagnosis of pancreatic cancer, in particular to the application of ZFPM2-AS1 in lncRNA in the preparation of a diagnostic kit for pancreatic related diseases. Background technique [0002] Pancreatic cancer is one of the most malignant tumors worldwide. Due to its insidious onset, early diagnosis is very difficult. About 80%-90% of patients have developed distant metastases when they see a doctor, and the surgical resection rate is only 15%, and the postoperative survival rate is not satisfactory. Despite the continuous advancement of medical technology, its mortality and survival rates have not changed significantly in the past decade. In addition, the incidence of pancreatic cancer has shown an upward trend in recent years both at home and abroad. Therefore, early diagnosis is the key to improve the resection rate and survi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6886C12Q1/6851
CPCC12Q1/6851C12Q1/6886C12Q2600/118C12Q2600/178C12Q2561/113C12Q2563/107
Inventor 孔凡扬邓萱孔祥毓李兆申杜奕奇朱建伟鲍一朱惠云王宇欣
Owner SHANGHAI CHANGHAI HOSPITAL
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