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A method for identifying Dalbergia cochinensis and Dalbergia australis species by fluorescence spectrum

A technology of Dalbergia cochinchinensis and Austenitia yellow, which is applied in the field of identification and identification of wood species, can solve the problems of difficult accurate identification of tree species and difficult identification of mahogany species, and achieve the effect of convenient detection process, high efficiency and simple operation process

Active Publication Date: 2020-07-03
NANJING INST OF PROD QUALITY INSPECTION
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the most common method for identifying wood species in the world is the technology of wood macroscopic and microscopic structure characteristics. Professional wood anatomy technicians can use the macroscopic characteristics of mahogany wood texture, color, and smell, as well as the conventional ray superposition, axial parenchyma, and attached The microscopic characteristics such as pits and tube hole arrangement were observed, and the identification and identification of mahogany species were carried out against the database of wood macroscopic and microstructural characteristics of the International Association of Wood Scientists (IAWA). However, for the above two species of Dalbergia It is difficult to accurately determine its species
[0003] The traditional identification method of wood species is wood macroscopic and microscopic structure characteristic technology. Wood anatomy professional technicians base on mahogany wood texture, color and luster, smell and other macroscopic characteristics as well as conventional ray superposition, axial parenchyma, appendage pits, The identification and identification of mahogany tree species is carried out by observation of microscopic features such as tube hole arrangement. Although this identification and identification method is currently the most widely used method in the world, the dilemma of this method is that it can only distinguish the genus of mahogany. It is difficult to realize the identification and identification of mahogany tree species

Method used

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  • A method for identifying Dalbergia cochinensis and Dalbergia australis species by fluorescence spectrum
  • A method for identifying Dalbergia cochinensis and Dalbergia australis species by fluorescence spectrum
  • A method for identifying Dalbergia cochinensis and Dalbergia australis species by fluorescence spectrum

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Experimental program
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Effect test

Embodiment 1

[0038] Establishment of extraction conditions for fluorescent substances in Dalbergia cochinchinensis and Dalbergia austenifolia:

[0039] The wood standard products of Dalbergia cochinchinensis and Dalbergia austenii were sampled and crushed, and the fluorescent substances were extracted from the pulverized Dalbergia cochinchinensis and Dalbergia austenifolia with a solvent to obtain an extract containing fluorescent substances, and then the fluorescent substance containing The extraction solution was irradiated under a 365nm ultraviolet lamp in a dark room to obtain a fluorescence spectrum; according to the fluorescence intensity of the fluorescence spectrum obtained by irradiating under a 365nm ultraviolet lamp, the extraction conditions of the fluorescent substances of Dalbergia cochinchinensis and Dalbergia austenii were determined;

[0040] The specific steps are as follows: sample and crush the collected Dalbergia cochinchinensis and Dalbergia austenifolia wood, weigh 0....

Embodiment 2

[0045] Having determined that acetonitrile is used to extract the fluorescent substance of Dalbergia cochinchinensis, the mixed reagent of ethyl acetate and acetonitrile (according to volume ratio calculation acetonitrile: ethyl acetate=0.1:1) extracts the fluorescent substance of Dalbergia austenifolia, the present invention It also verified different batches of Dalbergia cochinchinensis and Dalbergia austenii, that is, extracting fluorescent substances from Dalbergia cochinchinensis with acetonitrile, and extracting fluorescent substances from Dalbergia austenifolia with a mixed reagent of ethyl acetate and acetonitrile. Whether the fluorescent substances of Dalbergia cochinchinensis and Dalbergia austenii from different sources are consistent.

[0046] image 3 Fluorescent photographs (365nm light irradiation) of five different sources of Dalbergia cochinchinensis soaked in acetonitrile extraction reagent for 12 hours.

[0047] Figure 4 Fluorescence photographs (365nm li...

Embodiment 3

[0050] The present invention extracts the fluorescent substance of Dalbergia cochinchinensis with acetonitrile and the mixed reagent of ethyl acetate and acetonitrile (calculated according to the volume ratio of acetonitrile:ethyl acetate=0.1:1) to extract the fluorescent substance of Dalbergia austenifolia The stability analysis of the substance extracts was carried out: the fluorescence photos of each extract were detected 12h after extraction, 12h in 1 year and 12h in 2 years.

[0051] Figure 5 Fluorescence photos of different time periods of the extract obtained by extracting fluorescent substances from Dalbergia cochinchinensis with acetonitrile.

[0052] Image 6 Fluorescence photos of different time periods of the extract obtained by extracting the fluorescent substance of Dalbergia austeni with a mixed reagent of ethyl acetate and acetonitrile (calculated according to the volume ratio of acetonitrile:ethyl acetate=0.1:1).

[0053] From the results, it can be seen th...

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Abstract

The invention discloses a detecting method for identifying dalbergia cochinchinensis and dalbergia oliveri tree species. The method comprises the following steps of (1) determining fluorescence substance extraction conditions in dalbergia cochinchinensis and dalbergia oliveri; (2) obtaining fluorescence substances according to the extraction conditions of the fluorescence substances in the dalbergia cochinchinensis and dalbergia oliveri determined in the first step; performing fluorescence characteristic information determination on the extracted fluorescence substances by using a molecular fluorescence spectrometry technology; building a fluorescence characteristic spectrum library of the dalbergia cochinchinensis and dalbergia oliveri tree species; (3) performing comparison on the practically detected fluorescence characteristic spectrum and the fluorescence characteristic spectrum library of the dalbergia cochinchinensis and dalbergia oliveri tree species built in the step (2) for identifying the dalbergia cochinchinensis and the dalbergia oliveri. The dalbergia cochinchinensis and dalbergia oliveri tree species are accurately identified by obtaining the fluorescence compounds of the dalbergia cochinchinensis and dalbergia oliveri tree species, performing fluorescence characteristic information determination and combining a conventional wood microcosmic and macroscopical microstructure identification technology. The technology belongs to a more scientific and efficient wood tree species identification novel method.

Description

technical field [0001] The invention belongs to the technical field of identification and identification of wood tree species, in particular to a method for identification and identification of two types of redwood species, Dalbergia cochinchinensis and Dalbergia austenii, in particular, the use of molecular fluorescence spectrum technology to determine the amount and fluorescence of fluorescent substances in the two types of redwood species. characteristics are measured. Background technique [0002] Dalbergia cochinchinensis and Dalbergia austeni belong to the same genus of Dalbergia wood, and their traditional micro and macro microstructures are very similar. At present, the most common method for identifying wood species in the world is the technology of wood macroscopic and microscopic structure characteristics. Professional wood anatomy technicians can use the macroscopic characteristics of mahogany wood texture, color, and smell, as well as the conventional ray superp...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/64
CPCG01N21/64
Inventor 王金砖杜海慧刘彬梁峙赵颖峰赵越张玉洁吴海娟
Owner NANJING INST OF PROD QUALITY INSPECTION