A method for identifying Dalbergia cochinensis and Dalbergia australis species by fluorescence spectrum
A technology of Dalbergia cochinchinensis and Austenitia yellow, which is applied in the field of identification and identification of wood species, can solve the problems of difficult accurate identification of tree species and difficult identification of mahogany species, and achieve the effect of convenient detection process, high efficiency and simple operation process
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Embodiment 1
[0038] Establishment of extraction conditions for fluorescent substances in Dalbergia cochinchinensis and Dalbergia austenifolia:
[0039] The wood standard products of Dalbergia cochinchinensis and Dalbergia austenii were sampled and crushed, and the fluorescent substances were extracted from the pulverized Dalbergia cochinchinensis and Dalbergia austenifolia with a solvent to obtain an extract containing fluorescent substances, and then the fluorescent substance containing The extraction solution was irradiated under a 365nm ultraviolet lamp in a dark room to obtain a fluorescence spectrum; according to the fluorescence intensity of the fluorescence spectrum obtained by irradiating under a 365nm ultraviolet lamp, the extraction conditions of the fluorescent substances of Dalbergia cochinchinensis and Dalbergia austenii were determined;
[0040] The specific steps are as follows: sample and crush the collected Dalbergia cochinchinensis and Dalbergia austenifolia wood, weigh 0....
Embodiment 2
[0045] Having determined that acetonitrile is used to extract the fluorescent substance of Dalbergia cochinchinensis, the mixed reagent of ethyl acetate and acetonitrile (according to volume ratio calculation acetonitrile: ethyl acetate=0.1:1) extracts the fluorescent substance of Dalbergia austenifolia, the present invention It also verified different batches of Dalbergia cochinchinensis and Dalbergia austenii, that is, extracting fluorescent substances from Dalbergia cochinchinensis with acetonitrile, and extracting fluorescent substances from Dalbergia austenifolia with a mixed reagent of ethyl acetate and acetonitrile. Whether the fluorescent substances of Dalbergia cochinchinensis and Dalbergia austenii from different sources are consistent.
[0046] image 3 Fluorescent photographs (365nm light irradiation) of five different sources of Dalbergia cochinchinensis soaked in acetonitrile extraction reagent for 12 hours.
[0047] Figure 4 Fluorescence photographs (365nm li...
Embodiment 3
[0050] The present invention extracts the fluorescent substance of Dalbergia cochinchinensis with acetonitrile and the mixed reagent of ethyl acetate and acetonitrile (calculated according to the volume ratio of acetonitrile:ethyl acetate=0.1:1) to extract the fluorescent substance of Dalbergia austenifolia The stability analysis of the substance extracts was carried out: the fluorescence photos of each extract were detected 12h after extraction, 12h in 1 year and 12h in 2 years.
[0051] Figure 5 Fluorescence photos of different time periods of the extract obtained by extracting fluorescent substances from Dalbergia cochinchinensis with acetonitrile.
[0052] Image 6 Fluorescence photos of different time periods of the extract obtained by extracting the fluorescent substance of Dalbergia austeni with a mixed reagent of ethyl acetate and acetonitrile (calculated according to the volume ratio of acetonitrile:ethyl acetate=0.1:1).
[0053] From the results, it can be seen th...
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