Transcription factor chip kit and method for high throughput screening of target gene transcription factor

Abstract

The present invention relates to the technical field of medical bioassay, and the present invention provides a transcription factor chip kit and a method for high throughput screening of a target genetranscription factor by using the transcription factor chip kit, in the method, a target gene promoter region DNA fragment is applied as a probe to a transcription factor active chip to effectively compensate for shortcomings of short of specificity of the transcription factor active chip, the method is possible to screen the target gene transcription factor quickly and efficiently, and meanwhilethe problem of subjective selectivity and missing of potential transcription factors can be avoided.

Description

technical field [0001] The invention relates to the technical field of medical biological detection, and relates to a transcription factor chip kit and a method for high-throughput screening of target gene transcription factors using the transcription factor chip kit. Background technique [0002] Transcription factors are a class of proteins that can bind to the DNA sequence of a target gene and initiate the transcription of the target gene. The binding of transcription factors to target gene DNA depends on the transcription factor binding site (transcription factor binding site, TFBS) in the promoter region of the target gene, and the promoter region is generally located within 1000 bp upstream of the transcription start site of the gene. Transcription factors regulate gene expression by binding to TFBS in the gene DNA sequence. [0003] Studies have found that in many diseases, especially cancer, the gene expression profiles of diseased tissues are significantly differen...

AI Technical Summary

Problems solved by technology

Although this method reduces the screening range of target gene transcription factors to a certain extent, after screening the transcription factors with changing activity, it is still unable to focus on the transcription factors that regulate the target gene, and can only analyze the ones that can bind to the target gene according to the database. Transcription factors, and then verified by EMSA, ChIP experiments, still time-consuming and laborious

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