Preparation method and applications of epithelial cell sheet for treating corneal diseases

Abstract

The invention relates to a method for preparing a tissue engineering oral mucosal epithelial cell sheet, and specifically provides an oral mucosal epithelial cell sheet preparation method, which comprises: (a) oral mucosal tissue collection; (b) oral mucosal epithelial cell extraction; (c) oral mucosal fibroblast extraction; (d) preparation of epithelial cell culture matrix and culture scaffold; (e) co-culture of oral mucosal epithelial cells and oral mucosal fibroblasts; and (f) separating of prepared epithelial cell sheet from the co-cultured material.

Description

technical field The invention relates to the field of tissue engineering and biological products, in particular, the invention relates to a preparation method and application of an epithelial cell sheet for the treatment of corneal diseases (especially corneal and ocular surface diseases). Background technique The human cornea is divided into 5 layers, from front to back: epithelial cell layer, Bowman's membrane, stroma layer, Bowman's membrane and endothelial cell layer. The epithelial cell layer is the outermost layer with a thickness of about 50 microns, accounting for 10% of the thickness of the entire cornea, and is composed of 5-6 layers of cells. The epithelium of the peripheral part of the cornea is thickened, and the number of cell layers increases to 8-10 layers. There are 3 types of cells in the corneal epithelium: basal cells, wing cells, and squamous cells. Epithelial cells are connected by bridge bodies to form a dense plasma membrane. This dense and strong b...

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Problems solved by technology

However, for patients with ocular surface damage in both eyes, because there is no healthy limbal tissue available for sampling, it is impossible to carry out

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