Method for detecting genotype of genes related to cell cycle control

A cell cycle and detection method technology, applied in the field of molecular biology, can solve the problems of false positive phenomena that cannot be well checked and explained, and achieve the effect of strong repeatability and high detection accuracy

Active Publication Date: 2010-05-26
上海中优医药高科技股份有限公司
View PDF0 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

False positives in the detection of CCND1 and STK15 genes in pre

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for detecting genotype of genes related to cell cycle control
  • Method for detecting genotype of genes related to cell cycle control
  • Method for detecting genotype of genes related to cell cycle control

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0035] A genotype detection method for genes related to cell cycle regulation:

[0036] Step 1. Types of samples tested and sampling methods:

[0037] Scrape the left and right cheeks of the subject's mouth with sampling swabs 20 times each to collect oral mucosal epithelial cell samples;

[0038] Step 2. Extraction method of genomic DNA:

[0039] Using the silica gel adsorption method to extract the genomic DNA of each oral epithelial cell sample of the test subject, the time is 2 hours to 2.5 hours. After electrophoresis detection, a clear white band can be seen with the naked eye and enter the next step of detection;

[0040] Step 3, fluorescent quantitative PCR reaction:

[0041] Put each test sample into 2 reaction wells, and detect 2 sites at the same time, namely, cyclin (CCND1) rs9344, serine / threonine kinase 15 (STK15) s2273535, and add 2 DNA-free Template NTC blank control well;

[0042] Add reagents to each reaction well as a fluorescent quantitative PCR reactio...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention provides a method for detecting genotype of genes related to cell cycle control, which is characterized by including the following concrete steps: collecting the oral mucosa sample of the detected; extracting genomic DNA, carrying out genotyping and analyzing the result. The DNA to be detected in the invention can be obtained by extracting the oral mucosa epithelial cell of the detected and the sample collection can be carried out by employing oral mucosa epithelial cell collection method and related equipment. As far as the DNA extraction of the sample is concerned, the silica gel adsorption method can be employed to extract the DNA of the oral epithelial cell and measure purity and concentration of the extracted DNA. Two-locus Genotyping can be carried out by adopting the TaqMan-MGB technology. As proven by the experiment, accuracy rate of genotyping reaches more than 99%, while the repeatability thereof 100%.

Description

technical field [0001] The invention relates to a genotype detection method of genes related to cell cycle regulation, which is used for detecting the variation of CCND1 and STK15 genes, and belongs to the technical field of molecular biology. Background technique [0002] Large-sample Chinese population studies have confirmed that mutations in the cyclin CCND1 and serine / threonine kinase STK15 genes are common in the Chinese population. False positives in the detection of CCND1 and STK15 genes in previous detection methods cannot be well investigated and explained. Contents of the invention [0003] The purpose of the present invention is to provide a genotype detection method of cell cycle regulation-related genes with high accuracy. [0004] In order to achieve the above object, the technical solution of the present invention is to provide a genotype detection method for genes related to cell cycle regulation, characterized in that, the specific steps are: [0005] St...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C12Q1/68G01N21/64
Inventor 王校傅咏南
Owner 上海中优医药高科技股份有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap