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A kind of Yersinia pseudotuberculosis insecticidal protein and its coding gene and application

A technology of Yersinia, insecticidal protein, applied in application, insecticide, genetic engineering, etc.

Inactive Publication Date: 2020-05-05
NORTHWEST A & F UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no report on the insecticidal protein and its gene derived from Yersinia pseudotuberculosis

Method used

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  • A kind of Yersinia pseudotuberculosis insecticidal protein and its coding gene and application
  • A kind of Yersinia pseudotuberculosis insecticidal protein and its coding gene and application
  • A kind of Yersinia pseudotuberculosis insecticidal protein and its coding gene and application

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Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0028] The technical solutions of the present invention will be further described in detail below in conjunction with specific embodiments.

[0029] Construction of YpkA303 inducible expression vector

[0030] To obtain a new gene YpkA303, use specific primers to amplify YpkA303 from wild-type Yersinia pseudotuberculosis (Yersiniapseudotuberculosis YPIII) genomic DNA. The primer sequence is as follows

[0031] Forward primer YpkA303-F: 5'-CGCGGATCCATGAAACCGTTAGATCAGAATATTA-3'

[0032] Reverse primer YpkA303-R:5'-CCGCTCGAGTTAACATCCCTCCGCCACCTTTTGAG-3'

[0033] (1) Amplification of YpkA303 gene

[0034] Table 1 YpkA303 gene PCR amplification system

[0035]

[0036] PCR program:

[0037]

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PUM

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Abstract

The invention discloses yersinia pseudotuberculosis insecticidal protein as well as an encoding gene and application thereof, and belongs to the technical field of genetic engineering and biopesticide. The insecticidal protein is protein consisting of an amino acid sequence as shown in SEQ ID NO:1, or is protein which is obtained by substituting and deleting the amino acid sequence as shown in SEQID NO:1 or inserting one or more animal sequences and has the same activity. The invention also provides the encoding gene, an expression vector and engineering bacteria of the insecticidal protein.The invention also provides a method for separating and purifying the yersinia pseudotuberculosis insecticidal protein. The invention provides application of the protein to an insecticide agent as well as application of the encoding gene of the protein to transformation plants. The invention provides the encoding gene of the insecticidal protein. The insecticidal protein provided by the inventioncan be used for killing lepidoptera pests such as greater wax moth, cotton bollworm, asparagus caterpillar and asiatic corn borer and can improve the insecticidal ability of transgenic crops.

Description

technical field [0001] The invention belongs to the technical fields of genetic engineering and biopesticides, and relates to a Yersinia pseudotuberculosis insecticidal protein and its coding gene and application, in particular to an insecticidal protein derived from Yersinia pseudotuberculosis (Yersiniapseudotuberculosis) Toxin protein YpkA303 and its gene and application in biological control. Background technique [0002] One of the main agricultural disasters in my country is crop diseases and insect pests. It has the characteristics of various types, great impact, and frequent outbreaks. The scope and severity of its occurrence often cause heavy losses to my country's agricultural production. According to statistics, our country loses up to 50 billion catties of grain and 18 million tons of various economic crops every year due to pests and diseases. Common pests and diseases of crops in my country include: corn borer, cotton bollworm, locust, greater wax moth, beet ar...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K14/24C12N15/31A01N47/44A01P7/04C12P21/02C07K1/36C07K1/34C07K1/22C12R1/19
CPCA01N47/44C07K14/24
Inventor 沈锡辉潘君风宋莉徐磊张磊王瑶
Owner NORTHWEST A & F UNIV