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Salt-tolerant trait molecular marker for Corchorus and application thereof

A technology of jute and salt tolerance, which is applied in the determination/inspection of microorganisms, DNA/RNA fragments, recombinant DNA technology, etc., can solve the problems of slow progress in salt tolerance research of jute and low salt tolerance of jute

Inactive Publication Date: 2018-10-02
INST OF BAST FIBER CROPS CHINESE ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, the research progress of jute salt tolerance is slow, people's understanding of jute salt tolerance is still in the initial stage, there are few reports on the molecular mechanism of jute salt tolerance, and there are relatively few previous reports on jute salt tolerance

Method used

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  • Salt-tolerant trait molecular marker for Corchorus and application thereof
  • Salt-tolerant trait molecular marker for Corchorus and application thereof
  • Salt-tolerant trait molecular marker for Corchorus and application thereof

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Experimental program
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Embodiment

[0056] 1. Project background

[0057] GBS (Genotyping-by-sequencing) technology refers to genotyping by sequencing, and constructing SNP molecular markers by selecting appropriate restriction endonucleases combined with high-throughput population sequencing, which can be used for molecular marker development, ultra-high-density genetic map construction, Population genetic analysis, population GWAS analysis and other fields.

[0058] GBS technical route such as figure 1 As shown, it mainly includes three parts:

[0059] 1) Experimental part: Electronic enzyme digestion evaluation, select the appropriate endonuclease according to the species and scientific research purpose, build a library after the sample is qualified, and sequence on the HiSeq PE150 sequencing platform;

[0060] 2) Standard information analysis: After basic quality control, the original sequencing data is compared with the reference genome for variation detection and screening;

[0061] 3) Advanced informat...

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Abstract

The invention relates to the technical field of plant breeding, in particular to a salt-tolerant trait molecular marker for Corchorus and application thereof, wherein Scaffold number of mk7047 SNP (single-nucleotide polymorphism) site marker is AWUE01014583.1, the site on Scaffold is 47766, a basic group of a reference sequence is G, and a variant basic group is T. The mk7047 SNP site provided herein is applicable to the breeding of salt-tolerant varieties of Corchorus and is also applicable to the studies in terms of population genetic structure, genetic variation level and the like.

Description

technical field [0001] The invention relates to the technical field of plant breeding, in particular to a molecular marker and application of salt-tolerant traits in jute. Background technique [0002] Soil salinization has evolved into a global problem, one of the important factors leading to land desertification and farmland degradation, and has become a major crisis facing mankind. Soil salinization has seriously affected the growth and development of plants and has become one of the major abiotic stresses in nature. At present, the global saline-alkali land area has reached 950 million hm 2 . Soil salinization has become an important factor restricting food production and affecting food security. However, the contradiction between the rapid growth of population and the shortage of food is increasing day by day. It is considered to be a solution with double economic and ecological benefits to improve and utilize saline-alkali land by screening and cultivating new varie...

Claims

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Application Information

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IPC IPC(8): C12Q1/6895C12N15/11
CPCC12Q1/6895C12Q2600/13C12Q2600/156
Inventor 杨泽茂粟建光戴志刚唐蜻程超华许英刘婵陈基权谢冬微
Owner INST OF BAST FIBER CROPS CHINESE ACADEMY OF AGRI SCI