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The experimental method of MiR-210 in the verification of relevant targets in glioma

An experimental method, the technology of mir-210mimics, is applied in biochemical equipment and methods, material inspection products, microbial measurement/inspection, etc., which can solve the problem that miR-210 targets are not clear and cannot provide targets for glioma treatment And other issues

Inactive Publication Date: 2018-10-12
WUXI NO 2 PEOPLES HOSPITAL
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Problems solved by technology

However, as far as the current research level is concerned, there are still many problems that need to be solved urgently.
First of all, there are still relatively few studies on miR-210 in glioma. Secondly, the target of miR-210 in glioma is still unclear, and it cannot provide a target for the treatment of glioma.

Method used

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  • The experimental method of MiR-210 in the verification of relevant targets in glioma
  • The experimental method of MiR-210 in the verification of relevant targets in glioma

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Embodiment Construction

[0031] The preferred embodiments of the present invention will be described below in conjunction with the accompanying drawings. It should be understood that the preferred embodiments described here are only used to illustrate and explain the present invention, and are not intended to limit the present invention.

[0032] Such as Figure 1-2 1. Clinical specimen collection and cell line culture as shown

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Abstract

The invention discloses an experimental method of MiR-210 in the verification of relevant targets in glioma. The experimental method is characterized by comprising the following steps of: using a glioma cell line, respectively constructing a miR-210 overexpression cell group, a miR-210 low-expression cell group and an empty granulocyte group, detecting the effect of miR-210 on the downstream genethrough the real-time qPCR, western blotting experiment, and detecting the expression level of the target protein and gene. The relevant biological effects of miR-210 are determined by MTT, invasion and migration. And whether the targets are correct by the reporter gene is determined.

Description

technical field [0001] The invention relates to an experimental method for verifying related targets of MiR-210 in glioma. Background technique [0002] Human glioma is the most common tumor of the central nervous system, accounting for about 40%-50% of adult intracranial tumors. At present, surgery is mainly used in clinical practice, supplemented by radiotherapy, chemotherapy and other comprehensive treatments. However, the treatment effect of glioma patients (especially glioblastoma) is still unsatisfactory, and the mortality rate and recurrence rate are still high. It is one of the tumors with the worst prognosis. In recent years, although microneurosurgery techniques and related adjuvant treatment methods have been greatly improved, the treatment effect is still poor, which is mainly related to the rapid growth, infiltration, invasion and drug resistance of human glioma, but among them mechanism is unclear. Exploring the pathogenesis of glioma and clarifying the mech...

Claims

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Application Information

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IPC IPC(8): C12Q1/6886C12Q1/02G01N33/574G01N33/68
CPCC12Q1/6886C12Q2600/158C12Q2600/178G01N33/5029G01N33/5044G01N33/57407G01N33/68
Inventor 蔺玉昌苗增利徐幸秦琪珑
Owner WUXI NO 2 PEOPLES HOSPITAL
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