Embryo Boundary Recognition and Laser Trajectory Fitting Method

Abstract

The invention provides a method for embryo boundary recognition and laser trajectory fitting, comprising the following steps: (A1) extracting the characteristic pattern of the microscopic image of the embryo collected; (A2) analyzing the characteristic pattern to obtain the embryo's Geometric boundary line; (A3) comparing the preset laser drilling track route and the geometric boundary line, thereby deciding whether to perform path optimization; if yes, enter step (A4); if not, use the preset laser drilling Hole trajectory route; (A4) re-optimize the laser trajectory path to avoid injuring the embryo. The invention has the advantages of being able to evaluate the damage risk of the laser path to the embryo, reset the laser drilling track and the like.

Description

technical field [0001] The invention relates to embryo manipulation, in particular to a method for embryo boundary recognition and laser trajectory fitting. Background technique [0002] In the assisted reproductive system, in the operation of hatching embryo implantation, when using the laser membrane rupture instrument, the user first needs to determine the position of the hole to be drilled on the microscope image, and then select the appropriate laser drilling trajectory on the cell zona pellucida , the laser drilling trajectory is displayed as a narrow curve on the display, it will be difficult for the human eye to distinguish whether the laser aperture will touch the boundary of the embryonic cell membrane, and the laser trajectory selected far away from the cell may not achieve the expected effect , choose too close and easy to damage the cell membrane, embryonic cell samples are precious, and operation failure will not be recovered, so there are many uncertainties in...

AI Technical Summary

Problems solved by technology

[0002] In the assisted reproductive system, in the operation of hatching embryo implantation, when using the laser membrane rupture instrument, the user first needs to determine the position of the hole to be drilled on the microscope image, and then select the appropriate laser drilling trajectory on the cell zona pellucida , the laser drilling trajectory is displayed as a narrow curve on the display, it will be difficult for human eyes to distinguish whether the laser aperture will touch the boundary of the embryonic cell membrane, and the laser trajectory selected far away from the cell may not achieve the expected effect , the selection is too close and the cell membrane is easy to be damaged. The embryonic cell sample is precious, and the failure of the operation will not be recovered. Therefore, there are many uncertainties in the preset drilling trajectory completely relying on the human eye, which also greatly increases the operational risk of the experiment.

There is currently no specific implementation plan for the assessment and response to this risk

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