Sample quantifying unit and microfluidic chip comprising same

Abstract

The invention discloses a sample quantifying unit and a microfluidic chip comprising the same. The sample quantifying unit comprises a sample injecting tank, a quantifying tank and a sample treatmenttank, wherein the sample injecting tank is communicated with a sample injecting port of the microfluidic chip; the quantifying tank comprises a quantifying zone and a waste liquid zone which are communicated; the quantifying zone comprises a buffer zone, a quantifying subzone and a precipitating subzone which are sequentially communicated; the buffer zone is communicated with the sample injectingtank through a first capillary connecting pipe; the top of the quantifying subzone is communicated with the waste liquid zone; a waste liquid tank is connected to the bottom end of the waste liquid zone; a sample enters the quantifying subzone and the buffer zone from the sample injecting tank by way of buffer zone, and the excessive sample enters the waste liquid zone from the top of the quantifying subzone; the sample treatment tank is connected with the quantifying subzone through a siphon; the sample injecting tank, the quantifying tank and the sample treatment tank are communicated with the atmosphere. The sample quantifying unit can achieve accurate quantification of the sample and reduces the error, so that the detection efficiency and the detection accuracy of the microfluidic chipare significantly improved.

Description

technical field [0001] The invention relates to the field of analysis and detection, in particular to a sample quantitative unit and a microfluidic chip with it. Background technique [0002] An indispensable and important step in biochemical analysis is sample collection and loading. In quantitative analysis, the accuracy of the added sample volume directly affects the accuracy of the final test results, so the quantitative accuracy of samples is very important for quantitative biochemical analysis. Conventional large-scale analytical instruments, such as automatic biochemical analyzers and immune analyzers, sample collection and quantification are realized by plunger pumps controlling sampling needles to draw samples. This method has the disadvantages of insufficient precision for processing trace samples and high hardware requirements, so it is widely used in large-scale automated instruments, and is not suitable for integrated and portable biochemical analysis devices. ...

AI Technical Summary

Problems solved by technology

This method has the disadvantages of insufficient precision for processing trace samples and high hardware requirements, so it is widely used in large-scale automated instruments, and is not suitable for integrated and portable biochemical analysis devices

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