Double droplet digital PCR absolute quantitative detection Kit for European and American porcine reproductive and respiratory syndrome virus

Abstract

The invention discloses a double droplet digital PCR absolute quantitative detection kit for European and American porcine reproductive and respiratory syndrome virus. The primer probe combination comprises LV-F1R1P1 and VR2332-F1R1P1, wherein LV-F1R1P1 consists of LV-F1, LV-R1 and LV-P1, the LV-F1 is as shown in a sequence 1, LV-R1 is as shown in a sequence 2, and LV-P1 is as shown in a sequence 3; VR2332-F1R1P1 consists of VR2332-F1, VR2332-R1 and VR2332-P1, VR2332-F1 is as shown in a sequence 4, VR2332-R1 is as shown in a sequence 5, and VR2332-P1 is as shown in a sequence 6. The kit has an important application value on prevention and control of European and / or American porcine reproductive and respiratory syndrome viruses, and is favorable to epidemic situation control from the source and effective prevention of porcine epidemic large-scale outbreak.

Description

technical field

[0001] The invention belongs to the field of virus detection, and in particular relates to a kit for absolute quantitative detection of porcine reproductive and respiratory syndrome virus European type and American type double droplet digital PCR. Background technique

[0002] Porcine reproductive and respiratory syndrome (Porcine reproductive and respiratory syndrome, PRRS) is caused by porcine reproductive and respiratory syndrome virus (Porcine reproductive and respiratory syndrome virus, PRRSV). Characteristic highly contagious infectious disease. The disease broke out in the United States for the first time in 1987, and then appeared in Canada, Europe and Asia one after another. At present, it has become popular all over the world, causing huge economic losses every year. PRRS first appeared in my country in 1995, and then spread rapidly. In 2006, a pig "high fever disease" with high body temperature, high morbidity and high mortality broke out in my c...