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Method for detecting sensitization of cosmetic ingredients by ultra-weak biophotonic imaging system

A biophotonic and imaging system technology, which is used in material analysis by optical means, preparation of test samples, measurement devices, etc., can solve the problems of long time, high cost, and large individual differences of volunteers, and achieves simple and fast operation. Effect

Active Publication Date: 2021-08-27
SOUTH CENTRAL UNIVERSITY FOR NATIONALITIES
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  • Description
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  • Application Information

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Problems solved by technology

However, the detection process of the human patch test is time-consuming and requires a large number of volunteers to participate, and there are large individual differences among volunteers.
However, the currently popular in vitro alternative animal experiments, such as human cell line activation tests, still have shortcomings such as time-consuming and high cost in the research of predicting chemical sensitization.

Method used

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  • Method for detecting sensitization of cosmetic ingredients by ultra-weak biophotonic imaging system
  • Method for detecting sensitization of cosmetic ingredients by ultra-weak biophotonic imaging system
  • Method for detecting sensitization of cosmetic ingredients by ultra-weak biophotonic imaging system

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Embodiment

[0047] Example: Ultra-weak biophoton imaging system detects cosmetic allergies

[0048] 1. Experimental materials:

[0049] Human skin tissue, from the Chinese Brain Bank Center (CBBC)

[0050] 2. Experimental steps

[0051] S1, the pretreatment of material, comprises the following steps:

[0052] S11. Retrieve the skin on the back of the forearm from the donated body provided by the Red Cross. In a large petri dish filled with a large amount of PBS buffer, use a scalpel and a sharp blade to remove the subcutaneous fat, leaving only the dermis and epidermis as much as possible .

[0053] S12. After removing the fat, cut the tissue into multiple 0.5×0.5cm tissue blocks, put them in antifreeze solution and infiltrate them in a -4°C refrigerator, and then divide the infiltrated tissue blocks into 2.0ml decanters Spin round bottom cryovials and store in liquid nitrogen at -80°C.

[0054] S2, tissue block culture, comprising the following steps:

[0055] S21. Take out the req...

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Abstract

The invention discloses a method for detecting the sensitization of cosmetic ingredients through an ultra-weak biophoton imaging system, which comprises the following steps: S1, pretreatment of materials; S2, tissue block culture; S3, experimental group setting; S4, experimental preparation and Imaging system preheating; S5, positioning and photographing; S6, experimental imaging; S7, sequence image processing and data analysis. The invention uses ultra-weak biophoton imaging technology and immunohistochemical staining method to rapidly detect the changes in the biophoton radiation intensity of the skin tissue donated by the dead body after the culture is treated with DNCB, a common cosmetic sensitizer. The invention can quickly and accurately explore the sensitization of cosmetic ingredients, detect whether the cosmetic contains chemical ingredients that cause skin allergic damage, and has the characteristics of simple and fast operation.

Description

technical field [0001] The invention relates to the safety detection of cosmetics. An ultra-weak biophoton imaging system is used to detect the change of biophoton intensity of spontaneous radiation of human skin treated by cosmetics, so as to determine whether the cosmetics contain chemical components that can cause skin allergic damage. It belongs to the field of biomedical detection technology, and this technology can also be applied to the fields of life science research, medical clinical examination and environmental protection. Background technique [0002] The composition of most cosmetics is complex, and the common allergens in the ingredients include fragrances, flavors, preservatives, metal elements (lead, mercury, nickel, arsenic), surfactants, emulsifiers, hormones (corticosteroid hormones, estrogens) etc.), moisturizers, 1-chloro-2,4-dinitrobenzene (DNCB), natural plant ingredients, etc. The diversification and high usage rate of cosmetics have led to an increa...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/84G01N1/28G01N1/42
CPCG01N1/286G01N1/42G01N21/84G01N2001/2873
Inventor 戴甲培郑敏明李泽华肖芳艳孙燕
Owner SOUTH CENTRAL UNIVERSITY FOR NATIONALITIES
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