Sheep meat quality SNP (single nucleotide polymorphism) marker and liquid chip formed by same

A liquid-phase chip, sheep meat technology, applied in the direction of DNA / RNA fragment, recombinant DNA technology, microbial determination / inspection, etc., can solve problems such as defective sheep meat quality

Pending Publication Date: 2018-10-26
湖北多羔生物育种科技有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The use of SNP has achieved certain results in some of the above studies, but there are still defects in the quality of sheep meat, especially in the technology of SNP and liquid phase chip.

Method used

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  • Sheep meat quality SNP (single nucleotide polymorphism) marker and liquid chip formed by same
  • Sheep meat quality SNP (single nucleotide polymorphism) marker and liquid chip formed by same
  • Sheep meat quality SNP (single nucleotide polymorphism) marker and liquid chip formed by same

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Embodiment example

[0015] The implementation of the present invention mainly includes SNP site screening and the preparation and application of site primer sequences and liquid phase chips.

[0016] 1. SNP site determination

[0017] Using the resequencing data of the muscles of different parts of the sheep body (longissimus dorsi, intercostal muscle, biceps femoris, lower limb muscles) and sheep annotation files, combined with the characteristics and rules of the 50K length of the gene, quality control And selection, get 127 SNP marker loci, the determination of these loci also provides the basis for the following primer sequence and the making of liquid phase chip.

[0018] 2. Synthesis of Primers

[0019] 127 pairs of primers are based on the sequence information of SMS001~SMS127 in the attached table.

[0020] 3. Preparation of liquid-phase chip

[0021] The primer sequences corresponding to the above 127 SMS001-SMS127 were mixed into liquid micro-droplets and spotted. The liquid phase c...

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Abstract

The invention relates to the technology of biology, mainly employing SNP (single nucleotide polymorphism) and liquid chip technologies. Muscular tissue samples are taken as research objects, and 127 SNP label loci are obtained after procedure quality control of data. A liquid chip with label upstream and downstream primers is designed and composited. Serial numbers of the 127 SNP loci range from SMS001 to SMS127. By application of 127 SNP labels, detection accuracy is improved, detection time is shortened, and detection level is improved. By the liquid chip with the SNP marked primer sequence,strong technical support and guarantee are provided for cloning of livestock genes with important economic traits, early heterosis prediction, marker assisted selection, discovery of new drugs, disease diagnosis, breeding, new-species generation, research on stress resistance and variety verification.

Description

technical field [0001] The sheep meat quality SNP marker and the liquid phase chip of its composition relate to the field of molecular biology SNP technology and chips, especially SNP screening markers and the liquid phase chip of its composition. Background technique [0002] In today's society, with the improvement of people's living standards and the enhancement of health awareness, the requirements for dietary structure and nutrition are getting higher and higher. Especially the intake of protein, and meat products are the main source of protein. Mutton has the characteristics of low fat, low cholesterol, high protein, tender and juicy, unique flavor and easy digestion. One of the high-grade meat products has become one of the main sources of protein in the human diet, and has been proven to have good nutritional value. Its meat quality characteristics are consistent with the current consumer demand for nutritious meat, and have satisfactory eating quality. Mutton occ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6888C12N15/11C40B40/06
CPCC12Q1/6888C40B40/06C12Q2600/156
Inventor 付绍印刘永斌张文广王位何小龙祁云霞王标特日格勒刘斌白雪
Owner 湖北多羔生物育种科技有限公司
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