Combined automatic liquid inlet chromatographic device

Abstract

The invention discloses a combined automatic liquid inlet chromatographic device, comprising a chromatographic column, a spherical nozzle container, a speed regulation type peristaltic pump and a pressurizing pump, wherein the spherical nozzle container comprises a spherical main body and a cylindrical pipe fixedly connected with the spherical main body, the lower end of the cylindrical pipe is sealed, the top of the spherical main body is connected with an adaptor with an adjusting valve, and the speed regulation type peristaltic pump is connected with the adaptor by a rubber hose; the side face of the top of the spherical main body is connected with the pressurizing pump by a gas connecting hole; the bottom wall of the spherical main body abuts the upper end of the chromatographic column, the upper end of the cylindrical pipe is communicated with the spherical main body, the lower end of the cylindrical pipe is inserted into the chromatographic column, and plurality of liquid flow holes are formed in the wall of the cylindrical pipe. By adding an eluent into the chromatographic column via the liquid flow holes, damage of adding of the eluent to the surface of silica gel can be prevented; the problem of low elution velocity is solved by the pressurizing pump; automatic adding of the eluent can be realized by the speed regulation type peristaltic pump, and manpower is saved.

Description

technical field [0001] The invention relates to the technical field of column chromatography, in particular to a combined automatic liquid-feeding chromatography device. Background technique [0002] Chromatography technology, also known as column chromatography technology, is based on the principle that the components in the sample mixture have different distribution coefficients in the stationary phase and the mobile phase, and the components are separated through repeated distribution. It includes column chromatography, paper chromatography, thin layer chromatography, high performance liquid chromatography, etc. At present, column chromatography technology is an essential and commonly used important technology for separation and purification of mixtures in natural product laboratories, chemical laboratories, biological laboratories and related industrial production. Column chromatography mainly includes atmospheric pressure column chromatography, vacuum column chromatogra...

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Problems solved by technology

[0003] Chinese Patent No. CN201720131404.3 discloses a constant-pressure pressurized column chromatography device, including a chromatography column, a spherical sprue container and a plastic interface clip, and the liquid outlet at the bottom of the spherical sprue container is socketed and fixed on the top opening of the chromatography column Inside, the plastic interface clamp is clamped and fixed at the connection between the spherical sprue container and the chromatography column. The center of the top of the spherical sprue container is provided with a liquid injection port and a sealing plug is provided at the liquid injection port. The top of the spherical sprue container An air port is provided on the side and the air port is connected to a constant pressure air pump, and a cock is provided at the outlet at the bottom of the chromatography column. This utility model improves the storage capacity of the chromatography column by setting a spherical sprue container that is movably connected with the chromatography column. The amount of eluent, but when the developing agent is added to the chromatography column through the spherical sprue container, it will drop directly from above, which will cause certain damage to the silica gel surface in the chromatography column, such as partially denting the silica gel surface, etc. , the chromatographic effect is not good when the developing agent diffuses along this uneven silica gel surface

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