A method for simultaneous detection of terpenes, terpene alcohol isomers and terpene esters in beer
A technology for terpene alcohols and mesoterpenes, which is applied in the field of simultaneous detection of terpenes, terpene alcohol isomers and terpene esters in beer, can solve the problem that no terpene oxides are involved, no effective detection method is proposed, etc. problem, to achieve the effect of high degree of automation, wide application range and simple pre-processing
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Embodiment 1
[0022] Embodiment 1 beer sample detection
[0023] Exhaust the beer liquid, take 5mL and put it into a 20mL headspace bottle, add 2g NaCl to the headspace bottle, mix well, pre-warm the derivative liquid in an incubator at 50-65°C for 10-20min, and then shake it at a speed of 500- Under the conditions of 600rpm and extraction temperature of 50-65°C, insert the DVB / CAR / PDMS fiber extraction head into the headspace bottle containing the derivative solution at 2cm and extract for 60-80min. After extraction, the extract is sent to gas detection, where the specific gas phase conditions are:
[0024] The inlet temperature is 250°C, the inlet adopts splitless mode, the chromatographic column adopts two chromatographic columns, RTSTEK and RT-bDEXsm, used in series, the carrier gas condition is helium, the gas velocity is 1mL / min, and the chromatographic heating program is : Keep at 80°C for 400min, then raise the temperature at 2°C / min to 100°C and keep for 100min, then raise the tem...
Embodiment 2
[0027] Example 2 The setting of qualitative and quantitative ions
[0028] Setting of qualifier ions in the detection of trace substances: qualifier ions are generally ions with large mass-to-charge ratio and high response value. Ions with a small mass-to-charge ratio are not representative, and many substances can be fragmented. The high response value is to improve the detection limit and facilitate quantification. In short, the higher the response, the less likely it is to be disturbed. Quantitative ion generally selects the ion with the largest response value, if there is interference, the second highest ion can be selected. The interference of co-distillates can be reduced by automatic judgment of qualifier ion ratio. Q0 is a quantitative ion, and Q1, Q2, and Q3 are qualitative ions. The qualitative and quantitative ions selected in this embodiment are shown in Table 1.
[0029] Qualitative and quantitative ions of each substance in Table 1
[0030]
[0031]
Embodiment 3
[0032] The establishment of embodiment 3 standard curve
[0033] The standard concentration and manufacturer of each substance used in this example are shown in Table 2. The standard curve establishment method is as follows: individually weigh the standard substance of each substance into a 10mL brown volumetric flask, and dissolve it with absolute ethanol , constant volume, calculate the final solution concentration of each, and then mix and prepare each single standard according to the calculated volume to form the concentration gradient 4 in Table 3, and then gradually dilute to form other concentration gradients. See Table 3 and Table 3 (continued) for details.
[0034] Table 2 Standard concentrations and manufacturers of each substance
[0035]
[0036] Table 3 standard curve
[0037]
[0038]
[0039] Table 3 (continued)
[0040]
PUM
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