Marking method suitable for acanthopagrus schlegelii juveniles

A marking method and juvenile fish technology, applied in the fields of application, fish farming, animal husbandry, etc., to achieve the effect of low price, good marking quality and low toxicity

Inactive Publication Date: 2018-11-09
SOUTH CHINA SEA FISHERIES RES INST CHINESE ACAD OF FISHERY SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no patent report on fluorescent dyeing marking of black sea bream at home and abroad

Method used

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  • Marking method suitable for acanthopagrus schlegelii juveniles
  • Marking method suitable for acanthopagrus schlegelii juveniles
  • Marking method suitable for acanthopagrus schlegelii juveniles

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] The black sea bream juveniles provided in this example are 40-49 mm in length and 1.3-2.8 g in body weight, and they are temporarily raised in 500 L culture tanks 10 d at a temporary density of 1.6 fish / L. During the temporary raising period, the pellet feed (Yuan Brand 0# floating pellet feed) was fed twice a day in the morning and evening until full. During temporary cultivation, the water temperature was kept at 23±2.5°C, the salinity was 33±2.0‰, the dissolved oxygen was 6.22mg / L, the pH was 7.6-7.8, and the photoperiod was 14L / 10D. The daily water change rate accounts for 30% of the aquaculture water body.

[0027] Alizarin red (molecular formula: C 14 h 7 NaO 7 S·H 2 O) be ground into fine powder (alizarin red is orange-yellow or yellow-brown powder, soluble in water), with the cultivated water after filtering, through careful stirring, all dissolvings, make the mother liquor of 2000mg / L stand-by; During the preparation process, an appropriate amount of sodiu...

Embodiment 2

[0031] Refer to Example 1 for the temporary rearing of black sea bream juveniles provided in this example and the preparation of alizarin red dyeing solution mother solution.

[0032] The dipping container uses a holding tank with a capacity of 60L, and places it in a shaded and quiet place. Add filtered breeding water to the dipping container, and use the mother liquor to make 30L of 200mg / L dipping labeling solution, adjust the pH to 7.6, and the natural water temperature (23 ± 2.3 ℃). 60 black sea bream juveniles were randomly placed in each dipping bucket, and the air pump was used to inflate continuously, and the dipping time was 24 hours.

Embodiment 3

[0034] Refer to Example 1 for the temporary rearing of black sea bream juveniles provided in this example and the preparation of alizarin red dyeing solution mother solution.

[0035] The dipping container uses a holding tank with a capacity of 60L, and places it in a shaded and quiet place. Add filtered breeding water to the dipping container, make 300 mg / L dipping marking liquid with the mother liquor, adjust the pH to 7.6, and adjust the pH to 7.6. The natural water temperature (23 ± 2.3 ℃). 60 black sea bream juveniles were randomly placed in each dipping bucket, and the air pump was used to inflate continuously, and the dipping time was 24 hours.

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PUM

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Abstract

The invention relates to a marking method suitable for acanthopagrus schlegelii juveniles. The method includes the following steps: (1) preparing multiple to-be-marked acanthopagrus schlegelii juveniles with body lengths of 3-5 centimeters; (2) preparing a fluorescent labeling dye into a mother solution of 2,000 mg / L for later use; (3) continuously adding sodium bicarbonate to adjust the pH valueof the solution to 7.5-7.8 during a mother solution preparing process; (5) preparing multiple dipping containers with appropriate sizes; (6) preparing a dipping marking solution of 100-400 mg / L with the mother solution; (7) putting the acanthopagrus schlegelii juveniles with a density of 0.5 per / L in the dipping marking solution; and (8) using clean seawater to wash the dye liquor on the acanthopagrus schlegelii juveniles after the dipping finishes. The tracking technical problem of the acanthopagrus schlegelii juveniles during proliferation and releasing after the acanthopagrus schlegelii juveniles are released can be solved; and marking effects can be guaranteed by taking alizarin red as the fluorescent labeling dye, and thus, efficient, fast and safe dipping marking can be performed onthe acanthopagrus schlegelii juveniles, and marking quality can be guaranteed in a period of time.

Description

technical field [0001] The invention belongs to the technical field of tracking and monitoring of breeding and releasing, and in particular relates to a marking method capable of effectively marking juvenile black sea bream. Background technique [0002] Black sea bream (Acanthopagrus schlegelii) belongs to the order Perciformes, breamidae, and the genus Redfish. It is a common warm-water bottom economic fish in the coast of my country. It is distributed in waters with sandy mud and water depths of 40-60m. Its meat is delicious and grows rapidly. , strong adaptability, inhabits estuaries and bays, does not migrate long distances, and is an important target for catching fish and increasing breeding. Black sea bream is an island and reef fish, and its seed breeding technology is mature. It is a common release target in various coastal areas of my country, and it is also one of the main release targets in the South my country Sea. [0003] In order to better restore and protect...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K61/95
CPCA01K61/95
Inventor 刘岩孙典荣耿倩杨长平单斌斌刘胜男李腾刘蔓婷
Owner SOUTH CHINA SEA FISHERIES RES INST CHINESE ACAD OF FISHERY SCI
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