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Solid culture method for increasing polysaccharide content of inonotus obliquus

A solid-state culture, solid-state culture medium technology, applied in microorganism-based methods, biochemical equipment and methods, and adding compounds to stimulate growth, etc., can solve the problems of low product yield, slow growth rate, and low component ratio.

Active Publication Date: 2018-11-13
TIMING PHARMA
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  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

However, at the present stage, the solid-state culture technology of Chaga has lower product yield, slower growth rate, and the ratio of active ingredient polysaccharides contained is lower than that of naturally occurring wild Chaga

Method used

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  • Solid culture method for increasing polysaccharide content of inonotus obliquus
  • Solid culture method for increasing polysaccharide content of inonotus obliquus

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Experimental program
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Embodiment

[0037] Such as figure 1 As shown in step S100, in this embodiment, the chaga strain is first inoculated on the solid medium, wherein the solid medium is placed in a solid culture bottle, and the bottom area of ​​the solid culture bottle is 220 square centimeters. Furthermore, the aforementioned solid medium is preferably mixed with mung bean powder, glucose, birch wood chips, agar and water in the following ratio: 11.8% mung bean powder, 9.7% birch wood chips, 1.3% glucose, 9.5% agar And 67.7% of water, and prepared by high temperature sterilization. Next, the Chaga strain is cultured in a light environment at normal temperature to produce Chaga mycelium, wherein the normal temperature refers to controlling the temperature of the culture environment between 15°C and 25°C, and preferably 25°C.

[0038] Subsequently, if figure 1As shown in step S102, when the chaga mycelium is cultivated to an area between 50 cm2 and 100 cm2, and preferably 80 cm2, the brightness of the cultur...

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Abstract

The invention discloses a solid culture method for increasing polysaccharide content of inonotus obliquus. The solid culture method includes steps of inoculation, first culture environment adjustment,nutrient solution adding, second culture environment adjustment and harvesting. The solid culture method specifically includes: firstly, performing inoculation of an inonotus obliquus strain in a solid culture medium to culture inonotus obliquus mycelia; secondly, after the inonotus obliquus mycelia reach a first preset area, reducing brightness and temperature of a culture environment, and culturing for a period of culture time in a low-temperature shade environment; thirdly, after adding of nutrient solution, recovering brightness and temperature of the culture environment, and harvesting when the inonotus obliquus mycelia reach a second preset area. Therefore, the content of triterpenoids in products of inonotus obliquus mycelia can be effectively increased.

Description

technical field [0001] The invention relates to a cultivation method of Chaga, in particular to a cultivation method for increasing the content of polysaccharides in Chaga. Background technique [0002] Chaga (Inonotus obliquus) is a folk medicinal fungus mainly distributed in Russia and Eastern Europe. Its Chinese aliases include Chaga, Chaga, Inonotus obliquus, Siberian Ganoderma, Chaga and tree mushrooms. The scientific classification belongs to the kingdom Fungi, Basidiomycota, Agaricomycetes, Hymenochaetales, Hymenochaetaceae, and Inonotus. [0003] In recent years, studies have shown that the polysaccharides and triterpenoids contained in chaga can effectively activate the immune system and inhibit the proliferation of viruses, and have the effect of preventing breast cancer, liver cancer, uterine cancer, gastric cancer, diabetes and high blood pressure , its nutritional and medicinal value gradually arouse people's attention. [0004] The early use of chaga was main...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12N1/38C12R1/645
CPCC12N1/14C12N1/38
Inventor 王伯纶
Owner TIMING PHARMA