A kind of highly active probiotic solid beverage with anti-helicobacter pylori effect

A solid beverage and probiotic technology, applied to bacteria, lactobacillus, bifidobacteria and other directions used in food preparation, can solve the problem of less research on probiotic solid beverages, and achieve increased growth and reproduction rate and good heat resistance. Effect

Active Publication Date: 2019-05-31
ZHONGKE YIKANG BEIJING BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0013] Helicobacter pylori is a Gram-negative bacillus, which is the only cause of chronic active gastritis and peptic ulcer, and is closely related to gastric malignant tumors. It is listed as a class I carcinogen by the World Health Organization and the International Union Against Cancer , most of the probiotic solid beverages reported in the prior art can maintain the colony balance of the intestinal tract, but there are few studies on the probiotic solid beverages against Helicobacter pylori, so it is necessary to provide a The role of probiotics solid drink

Method used

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  • A kind of highly active probiotic solid beverage with anti-helicobacter pylori effect
  • A kind of highly active probiotic solid beverage with anti-helicobacter pylori effect

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0066] The preparation method of this highly active probiotic solid beverage is:

[0067] (1) Stir the formula dosage of Lactobacillus helveticus powder, Lactobacillus rhamnosus powder, Bifidobacterium animal powder, Bifidobacterium lactis powder, Lactobacillus acidophilus powder and Bifidobacterium bifidum powder After homogenization, mixture A is obtained;

[0068] (2) Mix inulin, resistant dextrin and mixture A, and mix well to obtain mixture B;

[0069] (3) Mix the obtained mixture B with the rest of the raw materials in the formula, and after stirring evenly, obtain the mixture D;

[0070] (4) The mixture obtained in step (3) is freeze-dried to obtain the probiotic solid beverage.

[0071] The stirring speed in step (1) is 5-15r / min, and the stirring time is 30-50min.

[0072] The stirring speed in step (2) is 20-30r / min, and the stirring time is 20-40min.

[0073] The rotating speed of stirring in the step (3) is 35-45r / min, and the time of stirring is 10-30min.

[...

experiment example 1

[0094] The high-activity probiotic solid beverages prepared in Examples 1-10 and Comparative Examples 1-7 were tested for in vitro inhibition of Helicobacter pylori, and the specific steps were as follows:

[0095] 1. Materials

[0096] The strain H.CagA(+) pylori is the international standard strain NCTC11637, which is CagA(+) and VacA(+).

[0097] 2. Method

[0098] In a sterile environment, weigh 5 g of the probiotic solid beverages of Examples 1-10 and Comparative Examples 1-7, dissolve them in 200 ml of sterile water, adjust the pH of the solution to 2.0 with hydrochloric acid, stir at 40°C for 2 hours, and filter , the filtrate was rotary evaporated to a volume of less than 50ml, and then dilute to 100ml with sterile water. Then it was diluted to 1:40. A number of circular filter paper pieces with a diameter of 6 mm were punched out with a filter paper puncher, and sterilized by high-pressure steam at 120°C. Under a sterile environment, use sterile tweezers to pick u...

experiment example 2

[0105] 1. Materials: Male mice are weighed, 10 in every group, according to the highly active probiotic solid beverage provided by the embodiment of the present invention 1-10, the mice are weighed, and the mice are weighed according to 1.67g / kg.bw respectively Practice blood gavage for 10 days, and the blank group uses sterile diluent to tank the stomach. Before administering the test substance, take 0.1 g of mouse feces, add 10-fold dilution with sterile normal saline, calculate the number of bacteria in each gram of wet stool, take the logarithm and perform statistics, after the last administration of the test substance 24 , take feces aseptically, and detect the intestinal flora, the method is the same as above, and the results are shown in Table 3:

[0106]

[0107] As can be seen from Table 3, the highly active probiotic solid beverage provided by Examples 1-10 of the present invention, after the mice were fed, the content of Bifidobacterium and Lactobacillus was high...

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Abstract

The object of the present invention is to provide a kind of highly active probiotic composition with the effect of resisting Helicobacter pylori, which comprises by weight: 40-60 parts of resistant dextrin, 5-15 parts of inulin, 5 parts of fructooligosaccharide ‑15 parts, 2‑8 parts of Lactobacillus helveticus powder, 2‑8 parts of Lactobacillus rhamnosus powder, 2‑8 parts of Bifidobacterium animal powder, 2‑8 parts of Bifidobacterium lactis powder, acidophilus Lactobacillus powder 2-8 parts and Bifidobacterium bifidum powder 2-8 parts. The components of the highly active probiotic solid beverage provided by the present invention interact with each other to promote gastrointestinal peristalsis, promote the balance of gastrointestinal flora, increase the result of the single action of each component, and can better eliminate Helicobacter pylori, increasing beneficial bacteria content.

Description

technical field [0001] The invention belongs to the technical field of food, in particular relates to a solid beverage and a preparation method thereof, and more particularly relates to a highly active probiotic solid beverage capable of resisting Helicobacter pylori. Background technique [0002] Probiotics, the word probiotics in English is derived from the Greek word "for live", translated as "for life". It can colonize the host and improve the micro-ecological balance of the host, thereby playing a beneficial role. Studies in recent years have shown that there are more than 1,000 kinds of bacteria in the human body, especially the bacteria in the gastrointestinal tract are the most abundant, accounting for about 80% of the total number of microecological bacteria in the whole body, which is 10 times that of human cells. Two Genomes". These bacteria help the body digest food, produce vitamins to prevent diseases caused by bacteria in food, and stimulate the immune syste...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12N1/16A23L2/39A23L2/52A23L33/00
CPCA23L2/39A23L2/52A23L33/00C12N1/16C12N1/20A23V2200/00A23V2400/147A23V2400/113A23V2400/175A23V2400/51A23V2400/515A23V2250/5062A23V2200/3204A23V2200/32A23V2200/324
Inventor 张烨刘海霞耿然
Owner ZHONGKE YIKANG BEIJING BIOTECH CO LTD
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