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Raman spectroscopy discriminating method for Lycium barbarum

A technology of Raman spectroscopy and Ningxia wolfberry, which is applied in the field of Raman spectrum identification of Ningxia wolfberry, can solve the problem that it is susceptible to fluorescence background interference, Raman spectrum characteristic peaks have no quantitative indicators, and manual peak-finding identification analysis is not easy for high-throughput and rapid identification To achieve the effects of reducing fluorescence background interference, improving identification procedures, and reliable identification methods

Inactive Publication Date: 2018-11-16
宁夏林业研究院股份有限公司
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Problems solved by technology

[0003] Prior art "Confocal Raman Spectroscopy Identification of Lycium barbarum" ("Spectrum Laboratory", Volume 21, No. 6, Pages 1211-1212, November 2004) through the Raman spectra of Lycium barbarum from different origins The comparison of the characteristic peaks achieved the purpose of identifying Ningxia wolfberry and northern wolfberry according to the difference of Raman group frequency vibration peaks. Peak discrimination analysis is not easy to achieve high-throughput rapid identification

Method used

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  • Raman spectroscopy discriminating method for Lycium barbarum
  • Raman spectroscopy discriminating method for Lycium barbarum
  • Raman spectroscopy discriminating method for Lycium barbarum

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Embodiment 1

[0036] Select 10 dried wolfberry samples from Ningxia, 10 dried fruit samples from Qinghai wolfberry, 10 dried fruit samples from Xinjiang wolfberry and 10 dried wolfberry samples from Gansu as the calibration sample set; the above 40 wolfberry samples were frozen in the refrigerator at -18°C for 12 hours and then taken out. Immediately pulverize the sample into powder with a small medicinal material pulverizer for use without sieving.

[0037] Using the inVia Basis confocal micro-Raman spectrometer from Renishaw (UK), the Raman spectra of 10 samples of Lycium barbarum from Ningxia and 30 samples of Lycium barbarum from other origins (Qinghai, Xinjiang, and Gansu) were sequentially collected as Ningxia Lycium barbarum and Lycium barbarum of other origins correction set. Test conditions: laser wavelength 785nm, objective lens 10×, measurement range 300-3000cm -1 , exposure time 10s, laser power 5%. The Raman spectrum of a typical Ningxia wolfberry powder sample is as follows:...

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Abstract

The invention relates to a Raman spectroscopy discriminating method for Lycium barbarum. The method comprises the following steps: 1) collecting a correction set: selecting at least ten dried Lycium barbarum samples, and using at least ten dried wolfberry fruit samples produced in other places as correction samples; 2) establishing a qualitative discriminating model; and 3) measuring a sample to be detected. The method is a method for discriminating the production place of Lycium barbarum by combining a stoichiometry technology with a Raman spectroscopy technology, completes the discriminatingprogram, and can scientifically and efficiently complete the qualitative discrimination of Lycium barbarum and wolfberry fruits from other production places.

Description

technical field [0001] The invention relates to a Raman spectrum identification method of Ningxia wolfberry. Background technique [0002] The main planting areas of wolfberry in my country are Ningxia, Qinghai, Gansu, Xinjiang, etc. Due to the influence of ecological environment and climate and other factors, there are differences in the content of nutrition and active ingredients of wolfberry in different production areas, so there are also differences in quality and efficacy. The most authentic and famous one is Ningxia wolfberry. The market demand of Ningxia wolfberry is very large, but the source is confusing and the authenticity is difficult to distinguish. In the wolfberry trade, wolfberries from other origins are often mixed and pretended to be Ningxia wolfberries, which not only disrupts the normal market order, but also damages the interests of consumers. Therefore, it is necessary to compare and identify the quality of Lycium barbarum from Ningxia and other place...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/65
CPCG01N21/65
Inventor 张轩
Owner 宁夏林业研究院股份有限公司
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