Chronic thromboembolic pulmonary hypertension rat model and building method thereof

A pulmonary arterial hypertension and rat model technology, applied in medical science, veterinary instruments, veterinary surgery, etc., can solve problems such as the inability to truly simulate the pathophysiological process of pulmonary thrombosis and the difficulty in establishing it

Inactive Publication Date: 2018-11-20
THE FIRST AFFILIATED HOSPITAL OF FUJIAN MEDICAL UNIV
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  • Claims
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Problems solved by technology

[0005] (1) Animals have high fibrinolytic ability and no spontaneous lower extremity deep vein thrombosis, and it is difficult to establish an ideal animal model for CTEPH
[0006] (2) There have been models of embolic pulmonary hypertension of different animal species such as pigs, dogs, and rats, but the embolic substances are non-thrombotic substances such as small glass beads, polystyrene microspheres, and polidocanol, which cannot be truly simulated The pathophysiological process similar to clinical CTEPH patients, such as the stimulation of blood vessels by pulmonary thrombus

Method used

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  • Chronic thromboembolic pulmonary hypertension rat model and building method thereof
  • Chronic thromboembolic pulmonary hypertension rat model and building method thereof
  • Chronic thromboembolic pulmonary hypertension rat model and building method thereof

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Embodiment Construction

[0023] In order to make the object, technical solution and advantages of the present invention more clear, the present invention will be further described in detail below in conjunction with the examples. It should be understood that the specific embodiments described here are only used to explain the present invention, not to limit the present invention.

[0024] Carrageenin (carrageenin) is a polysaccharide that has a strong inflammatory effect and is widely accepted for inducing various inflammatory models. Multiple internal jugular vein injections of autologous thrombus combined with intraperitoneal injections of carrageenin and methylcycline Acid-resistant fibrinolytic therapy can ensure that the thrombus is stably stored in SD rats for a long period of time, and secondary new thrombus forms, continuously and stably stimulates the endothelium, causes endothelial remodeling, and builds an ideal, pathophysiological and clinical CTEPH patient. A rat model of chronic thromboe...

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Abstract

The invention belongs to the technical field of a new variety of vertebrates, and discloses a chronic thromboembolic pulmonary hypertension rat model and a building method thereof. Chloral hydrate ata concentration of 10 percent is injected through the abdominal cavity for anesthesia; under a sterile condition, through a left internal jugular vein, 1.5 mL of tranexamic acid-containing normal saline mixed with about 32 + / - 5 autologous thromboemboli embolizes the pulmonary artery at a rate of 0.5 ml / min; the rat is slowly turned in the embolizing process to enable a larger number of emboli toenter the lung circulation of the rat; after being embolized, the rat has cyanosis and breathes faster; on the fourth day and the eleventh day of the embolization, the rat is embolized for the secondtime and the third time according to the same method; the rat is observed for 5 weeks. The CTEPH model built by the building method disclosed by the invention takes the autologous emboli of the rat asemboli to embolize the pulmonary artery and enables the autologous emboli to embolize a blood vessel as long as possible under the help of pro-inflammatory effect of carrageenin, so as to really model the whole pathophysiological process of pulmonary embolism secondary CTEPH of a clinical CTEPH patient.

Description

technical field [0001] The invention belongs to the technical field of new varieties of vertebrates, and in particular relates to a chronic thromboembolic pulmonary hypertension rat model and a construction method thereof. Background technique [0002] At present, the existing technologies commonly used in the industry are as follows: [0003] Chronic thromboembolic pulmonary hypertension (CTEPH) is acute pulmonary embolism (APE) or pulmonary artery thrombosis in situ, the thrombus persists due to various reasons without dissolution, through organization, fibrosis, severe Consequences of pulmonary vascular obstruction. Although the incidence of CTEPH is low, the prognosis is very poor. Continuously elevated pulmonary vascular resistance will eventually lead to the development of right heart failure and death. Research data show that the level of pulmonary artery pressure is closely related to the prognosis. Currently, pulmonary artery thromboendarterectomy (Pulmonary End...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61D1/00
CPCA61D1/00
Inventor 邓朝胜
Owner THE FIRST AFFILIATED HOSPITAL OF FUJIAN MEDICAL UNIV
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