New Application of Rosaside

A technology of rosin, toxic and side effects, applied in the field of medicine, can solve the problem of difficult to effectively treat cancer, and achieve the effects of improving cell survival rate, enhancing physique, and prolonging survival period

Active Publication Date: 2021-01-26
广西英路维特药物有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Surgery and radiotherapy are local treatments, which are only effective for tumors at the treatment site. For potential metastatic lesions (cancer cells have actually metastasized, but cannot be found and detected clinically due to the limitations of current technical means) and clinical metastases cancers are difficult to treat effectively
There are no reports on the effects of rosin on the toxic side effects of cytotoxic chemotherapy drugs

Method used

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  • New Application of Rosaside
  • New Application of Rosaside
  • New Application of Rosaside

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] Example 1. Identification of cell proliferation activity

[0040] 1. Cell Culture

[0041] Both human embryonic kidney cells (HEK-293T) and mouse sarcoma cells (S180) were treated with RPMI-1 640 complete medium containing 10% fetal bovine serum (containing 100 μg / ml streptomycin and 100 IU / ml penicillin) , placed at 37°C, 5% CO 2 Cultured in a constant temperature incubator. The cells were digested with digestive solution (0.25% trypsin) and then passaged. The cells were passaged for more than three generations, and the cells in good condition were taken for future use.

[0042] 2. MTT colorimetric method to detect cell proliferation activity

[0043] 2.1 Establishment of cisplatin, doxorubicin, paclitaxel and camptothecin injury model

[0044] (1) Take HEK-293T cells in the logarithmic growth phase, adjust the concentration of the cell suspension to 1×10 with RPMI-1640 complete medium containing 10% fetal bovine serum 5 cells / mL, mix well and inoculate in 96-well...

Embodiment 2

[0064] Example 2. Morphological changes of HEK-293 cells observed by inverted microscope

[0065] Take HEK-293T cells in the logarithmic growth phase, adjust the cell suspension concentration to 2×10 with RPMI-1640 complete medium containing 10% fetal bovine serum 5 cells / mL, inoculate in a 6-well plate after mixing, 2 mL / well, in 5% CO 2 , Cultured in a 37°C incubator. After the cells were cultured for 24 hours, the experiments were performed in random groups. The groups were divided into: cisplatin damage model group (100 μM); cisplatin + rosin group (100 μM + 50 μM, 100 μM + 100 μM), rosin was given 1 hour in advance; normal control group, the same volume of medium was added. Each group had 3 replicate wells, in 5% CO 2 , Continue culturing in a 37°C incubator. After culturing for 24 hours, observe the changes of cell morphology in each group under an inverted microscope, take pictures with Olympus Image-ProPlus6.0 software and conduct comparative analysis. The result ...

Embodiment 3

[0067] Example 3. The effect of rosin on the survival rate of balb / c mice induced by cisplatin

[0068] Experimental method: 8-week-old Balb / c male mice were randomly divided into four groups, and the grouping conditions and treatments were as follows: ① Control group (n=10): hydroxypropyl-β-cyclodextrin solution was given by intragastric administration every day ; ② Cisplatin model group (n=12): one-time intraperitoneal injection of cisplatin (20 mg·kg -1 ), giving hydroxypropyl-β-cyclodextrin solution by intragastric administration every day, continuously administering for 6 days, and observing the death situation of animals every day; Cisplatin (20mg·kg -1 ), intragastric administration of hydroxypropyl-β-cyclodextrin-included rosin (6 mg·kg -1 ) was administered continuously for 6 days, and the animal death situation was observed every day; ④ cisplatin+high dose rosaside group (n=12): one-time intraperitoneal injection of cisplatin (20mg·kg -1 ), intragastric administra...

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Abstract

The invention belongs to the technical field of medicine, and the invention provides a new application of rosin, especially the application of rosin in the preparation of drugs for reducing the toxic and side effects of cytotoxic chemotherapeutic drugs. Experiments show that rosin can improve the cell survival rate of cells damaged by cytotoxic chemotherapy drugs, improve the cell morphology of cells damaged by cytotoxic chemotherapy drugs, and improve the organ damage caused by cytotoxic chemotherapy drugs. At the same time, the combined use of rosin and cytotoxic chemotherapeutic drugs will not weaken the antitumor efficacy of cytotoxic chemotherapeutic drugs. It shows that rosin can reduce the toxic and side effects of cytotoxic chemotherapy drugs, improve the tolerance of cancer patients to chemotherapy, improve the quality of life of cancer patients, enhance their physical fitness, and prolong their survival.

Description

technical field [0001] The invention belongs to the technical field of medicine, and specifically relates to the new application of rosin, especially the application of rosin in the preparation of drugs for reducing the toxic and side effects of cytotoxic chemotherapy drugs. Background technique [0002] The Tibetan medicine Juema is the enlarged root tuber of Potentilla anserina L. in the family Rosaceae (Potentilla L.), also known as ginseng fruit. Its wild resources are mainly distributed in the western regions of my country, with the best quality in Gansu, Qinghai, Tibet and other producing areas. Fern hemp has a sweet taste and good nature. It has the effects of promoting body fluid and quenching thirst, invigorating the spleen and stomach, nourishing qi and blood, and has great nutritional and medicinal value. [0003] Modern pharmacological studies have shown that Bracken has anti-oxidation, anti-aging, anti-virus, anti-hyperlipidemia, liver protection and immune reg...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K31/7024A61P39/02A61P35/00A61K33/243A61K31/337A61K31/4745A61K31/704
CPCA61K31/337A61K31/4745A61K31/7024A61K31/704A61K33/24A61P35/00A61P39/02A61K2300/00
Inventor 刘艳丽康乃馨许琼明李笑然杨世林
Owner 广西英路维特药物有限公司
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