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Carrier used for fixing jellyfish nematocysts, preparation method and application thereof

A nematocyst and carrier technology, applied in the field of carrier for fixing jellyfish nematocysts and its preparation, can solve the problems that nematocysts are easy to drift out of the field of vision and easily irritate nematocysts, and achieve strong operability and equipment The effect of simple and fast preparation method

Active Publication Date: 2018-11-20
INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, most studies on the release of nematocysts are directly observing the nematocysts in the slide under a microscope. During the pressing process of the slides, it is easy to stimulate the release of nematocysts. Observed under an inverted microscope, nematocysts tend to drift out of the field of view

Method used

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  • Carrier used for fixing jellyfish nematocysts, preparation method and application thereof
  • Carrier used for fixing jellyfish nematocysts, preparation method and application thereof
  • Carrier used for fixing jellyfish nematocysts, preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] 1) Weigh 5.0 g of gelatin particles and place them in a beaker, add 20 mL of distilled water, and cover the beaker with a glass petri dish to prevent water vapor from escaping during heating.

[0022] 2) Heat the beaker in a 70°C water bath.

[0023] 3) After the gelatin particles are melted, stir gently with a glass rod to mix the gelatin and water thoroughly and without bubbles in the gelatin. Use a pipette to add 1 mL of gelatin to the 12-well plate while it is hot. It is a carrier with a thickness of 0.3 cm. Such as figure 1 Shown.

Embodiment 2

[0025] 1) Weigh 5.0 g of gelatin particles and place them in a beaker, add 20 mL of distilled water, and cover the beaker with a glass petri dish to prevent water vapor from escaping during heating.

[0026] 2) Heat the beaker in a 70°C water bath.

[0027] 3) After the gelatin particles have melted, stir gently with a glass rod to mix the gelatin and water thoroughly and without bubbles in the gelatin. Use a pipette to add 1.5 mL of gelatin to the 12-well plate while it is hot. After cooling and solidification, It is a gelatin carrier with a thickness of 0.4 cm.

Embodiment 3

[0029] 1) Weigh 4.0 g of gelatin particles into a beaker, add 20 mL of distilled water, and cover the beaker with a glass petri dish to prevent water vapor from escaping during heating.

[0030] 2) Heat the beaker in a 70°C water bath.

[0031] 3) After the gelatin particles are melted, stir gently with a glass rod to mix the gelatin and water thoroughly and without bubbles in the gelatin. Use a pipette to add 1 mL of gelatin to the 12-well plate while it is hot. It is a gelatin carrier with a thickness of 0.3 cm.

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Abstract

The invention relates to the technical field of marine organisms, and particularly relates to a carrier used for fixing jellyfish nematocysts, a preparation method and the application thereof. The carrier adopts gelatin with the concentration of 20% to 25% (w / v). The carrier is provided for researching the influence factor of the release of the jellyfish nematocysts, and the preparation method ofthe carrier is simple and easy in operation.

Description

Technical field [0001] The invention relates to the technical field of marine biology, in particular to a carrier for fixing the jellyfish barbed wire capsule and a preparation method and application thereof. Background technique [0002] In recent years, the number of jellyfish has increased sharply, and incidents of jellyfish stings have occurred frequently, seriously affecting coastal tourism, marine fishing, marine sports, and naval training. Jellyfish stings have become the most common marine life injury. According to estimates by the National Institutes of Health, approximately 150 million people are stung by jellyfish every year (Montgomery, L., Seys, J., Mees, J. To Pee, or Not to Pee:A Review on envenomation and treatment in European jellyfishspecies.Marine Drugs,2016,14,127.), among which there are many deaths; along the coast of Hawaii in the United States, about 500,000 people are stung by jellyfish each year; about 10,000 people are stung by jellyfish in Australia ea...

Claims

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Application Information

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IPC IPC(8): C12N11/02
CPCC12N11/02
Inventor 于华华李翱宇李荣锋陈晓琳刘松邢荣娥李鹏程
Owner INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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