Gene sequence construct for treatment of central nervous system diseases
A technology of the central nervous system and gene sequence, which is applied in nervous system diseases, gene therapy, genetic engineering, etc., can solve problems such as expression imbalance, achieve the effect of improving balance and improving treatment effect
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[0049] Examples:
[0050] One, such as figure 2 As shown, the construction of various constructs:
[0051] KL0039 vector, synthetic CMV enhancer-synapsin promoter-AADC-P2A-GCH1-P2A-TH and AADC-SV40 promoter-TH-PGK promoter-GCH1 sequence (where TH is a truncated form of TH); among them, CMV enhancer- synapsin promoter-AADC-P2A-GCH1-P2A-TH is connected to the pUC57 vector (pUC57-synapsin-AGT); the KL0039 vector here is a lentiviral transfer vector, selected from existing lentiviral vectors or based on needs Partially modified lentiviral vector.
[0052] 1. PD1 vector construction
[0053] Using KL0039 vector as a template, the sequence between WPRE and cPPT was amplified with Age-F+Sal-R, and the PCR product was recovered and purified by electrophoresis. The primer sequence used was Age-F: CTGAGTGCCATTGGATGA caatcaacctctggattaca; Sal-R: gattactattaataactactcacgcatgctcttctcca. At the same time, the pUC57-synapsin-AGT plasmid was digested with AgeI and SalI to recover a 4.1kb band; th...
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