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Method for efficiently producing L-proline

A proline and high-efficiency technology, applied in the field of high-efficiency production of L-proline, can solve the problems of disrupting cell physiological metabolism, changing the physical and chemical properties of fermentation liquid, and unstable osmotic pressure, so as to achieve the effect of increasing production and eliminating repression

Active Publication Date: 2018-11-20
WUXI JINGHAI AMINO ACID
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

In the fermentation production process of L-proline, the intermittent feeding process is usually used to eliminate the inhibition of high concentration glucose on the synthesis of L-proline; however, the intermittent feeding process is easy to cause drastic changes in the glucose concentration, causing Changes in physical and chemical properties, such as instantaneous large-scale dilution of fermentation broth, unstable osmotic pressure, etc., will disrupt the physiological metabolism of cells, thereby negatively affecting cell growth and metabolism and final fermentation production

Method used

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  • Method for efficiently producing L-proline

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] Embodiment 1: batch fermentation (DO is controlled at 10%) based on DO-stat feeding control strategy

[0050] The starting condition of the fermenter is the same as that of intermittent fed-batch fermentation. When the DO rises to 10%, the glucose is fed, and the DO-related feeding is controlled. Whenever the DO is higher than 10%, a short feeding is performed to maintain the dissolved oxygen at About 10% (the feeding liquid composition is 500g / L glucose), ferment for 58h, and detect the average yield and production intensity of L-proline.

[0051] The test results are: the average output of L-proline is 56.2g / L, and the production intensity is 0.97g / L·h.

Embodiment 2

[0052] Embodiment 2: Batch fermentation (DO is controlled at 20%) based on DO-stat fed-batch control strategy

[0053] The starting condition of the fermenter is the same as that of intermittent fed-batch fermentation. When the DO rises to 20%, the glucose is fed, and the DO-related feeding is controlled. Whenever the DO is higher than 20%, the fed-batch is carried out for a while to maintain the dissolved oxygen. At about 20% (the feed liquid composition is 500 g / L glucose), ferment for 58 hours, and detect the average yield and production intensity of L-proline.

[0054] The test results are: the average output of L-proline is 58.6g / L, and the production intensity is 1.00g / L·h.

Embodiment 3

[0055] Embodiment 3: batch fermentation (DO is controlled at 25%) based on DO-stat fed-batch control strategy

[0056] The starting condition of the fermenter is the same as that of intermittent fed-batch fermentation. When the DO rises to 25%, it starts to feed glucose, and controls DO-related feeding. Whenever DO is higher than 30%, it will be fed for a while to maintain dissolved oxygen. At about 30% (the feed liquid composition is 500 g / L glucose), ferment for 57 hours, and detect the average yield and production intensity of L-proline.

[0057] The test results are: the average output of L-proline is 61.5g / L, and the production intensity is 1.08g / L·h.

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Abstract

The invention discloses a method for efficiently producing L-proline, and belongs to the technical field of fermentation engineering. In the method provided by the invention, a DO-stat method is adopted to maintain the glucose concentration at a relatively low constant level, thus effectively eliminating a repression effect of glucose in a fermentation production process of L-proline; and comparedwith a batch fed fermentation process, the method can increase the average yield of L-proline from 77.4g / L to 84.3g / L, and the average production intensity from 1.84g / L.h to 2.11g / L.h, so that the method has a good industrial application prospect.

Description

technical field [0001] The invention relates to a method for efficiently producing L-proline, which belongs to the technical field of fermentation engineering. Background technique [0002] L-proline is a neutral amino acid containing an imino group, and one of the 20 basic amino acids that make up proteins. It is a non-essential amino acid for the human body. L-proline has a certain physiological activity, and has a wide range of uses in medicine, agriculture, chemical industry and food, etc., especially in medicine, it is an intermediate in the synthesis of many new drugs. [0003] At present, the industrial production of L-proline mainly adopts fermentation method. In the fermentation production process of L-proline, the intermittent feeding process is usually used to eliminate the inhibition of high concentration glucose on the synthesis of L-proline; however, the intermittent feeding process is easy to cause drastic changes in the glucose concentration, causing Change...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P13/24C12R1/15
CPCC12P13/24
Inventor 宁健飞陈晓双蔡立明
Owner WUXI JINGHAI AMINO ACID
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