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A method for producing l-proline

A technology for producing proline and producing strains is applied in the field of fermentation engineering, which can solve the problems of changing the physical and chemical properties of fermentation liquid, disturbing the physiological metabolism of cells, and affecting the fermentation production of cell growth metabolism, so as to achieve the effect of eliminating the inhibitory effect and improving the yield.

Active Publication Date: 2021-05-28
WUXI JINGHAI AMINO ACID
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the fermentation production process of L-proline, the intermittent feeding process is usually used to eliminate the inhibition of high concentration glucose on the synthesis of L-proline; however, the intermittent feeding process is easy to cause drastic changes in the glucose concentration, causing Changes in physical and chemical properties, such as instantaneous large-scale dilution of fermentation broth, unstable osmotic pressure, etc., will disrupt the physiological metabolism of cells, thereby negatively affecting cell growth and metabolism and final fermentation production

Method used

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  • A method for producing l-proline

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] Example 1: Batch fermentation based on DO-stat fed-batch control strategy (DO controlled at 10%)

[0050] The starting condition of the fermenter is the same as that of intermittent fed-batch fermentation. When DO rises to 10%, glucose is fed, and DO-related feeding is controlled. Whenever DO is higher than 10%, it is fed for a while to maintain dissolved oxygen at About 10% (the feed liquid composition is 500g / L glucose), ferment for 58 hours, and detect the average yield and production intensity of L-proline.

[0051] The test results are: the average output of L-proline is 56.2g / L, and the production intensity is 0.97g / L·h.

Embodiment 2

[0052] Example 2: Batch fermentation based on DO-stat fed-batch control strategy (DO controlled at 20%)

[0053] The starting condition of the fermenter is the same as that of intermittent fed-batch fermentation. When DO rises to 20%, glucose is fed, and DO-related feeding is controlled. Whenever DO is higher than 20%, it is fed for a while to maintain dissolved oxygen. At about 20% (the feed liquid composition is 500g / L glucose), ferment for 58 hours, and detect the average yield and production intensity of L-proline.

[0054] The test results are: the average output of L-proline is 58.6g / L, and the production intensity is 1.00g / L·h.

Embodiment 3

[0055] Example 3: Batch fermentation based on DO-stat fed-batch control strategy (DO controlled at 25%)

[0056] The starting condition of the fermenter is the same as that of intermittent fed-batch fermentation. When the DO rises to 25%, the glucose is fed, and the DO-related feeding is controlled. Whenever the DO is higher than 30%, the fed-batch is carried out for a while to maintain the dissolved oxygen. At about 30% (the feeding liquid composition is 500g / L glucose), ferment for 57h, and detect the average yield and production intensity of L-proline.

[0057] The test results are: the average output of L-proline is 61.5g / L, and the production intensity is 1.08g / L·h.

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Abstract

The invention discloses a method for producing L-proline, which belongs to the technical field of fermentation engineering. The present invention uses the DO-stat method to maintain the glucose concentration at a lower constant level, effectively eliminating the repression of glucose in the L-proline fermentation production process; The average yield of proline increased from 77.4g / L to 84.3g / L, and the average production intensity increased from 1.84g / L·h to 2.11g / L·h, which has a good industrial application prospect.

Description

technical field [0001] The invention relates to a method for producing L-proline, which belongs to the technical field of fermentation engineering. Background technique [0002] L-proline is a neutral amino acid containing an imino group, and one of the 20 basic amino acids that make up proteins. It is a non-essential amino acid for the human body. L-proline has a certain physiological activity, and has a wide range of uses in medicine, agriculture, chemical industry and food, etc., especially in medicine, it is an intermediate in the synthesis of many new drugs. [0003] At present, the industrial production of L-proline mainly adopts fermentation method. In the fermentation production process of L-proline, the intermittent feeding process is usually used to eliminate the inhibition of high concentration glucose on the synthesis of L-proline; however, the intermittent feeding process is easy to cause drastic changes in the glucose concentration, causing Changes in physica...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P13/24C12R1/15
CPCC12P13/24
Inventor 宁健飞陈晓双蔡立明
Owner WUXI JINGHAI AMINO ACID
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