Mercury ion detection product, method and smart phone imaging analysis system

A technology for detecting images and lines, used in material analysis by optical means, analysis of materials, analysis by chemical reactions of materials, etc.

Active Publication Date: 2018-11-20
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the current lateral flow chromatography sensors can only achieve qualitative or semi-quantitative detection, and additional special instruments are required for quantitative detection, and there is no simple and convenient way to directly read quantitative detection data from lateral flow chromatography sensors. Technical or Portable Instruments

Method used

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  • Mercury ion detection product, method and smart phone imaging analysis system
  • Mercury ion detection product, method and smart phone imaging analysis system
  • Mercury ion detection product, method and smart phone imaging analysis system

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0059] Example 1. Preparation of lateral flow chromatography sensor based on nucleic acid base mismatch

[0060] (1) Design of nucleotide sequence for detection

[0061] Sequence 1 (nucleotide sequence on gold nanoparticles): 5'-ThioMC6-GGTGGTGGTGGTGG-3'

[0062] Sequence 2 (nucleotide sequence on detection line): 5'-Biotin-CCCCCCCTCCTCCTCCTCC-3'

[0063] Sequence 3 (nucleotide sequence on quality control line): 5'-Biotin-CCCCCCCACCACCACCACCC-3'

[0064] All the nucleotide sequences designed above were obtained by artificial synthesis. Wherein, sequence 1 is obtained by modifying the 5' end of the nucleotide sequence shown in SEQID No.: 1 in the sequence listing with ThioMC6; sequence 2 is obtained by modifying the 5' end of the nucleotide sequence shown in SEQID No.: 2 in the sequence listing Sequence 3 is obtained after biotin-labeling the 5' end of the nucleotide sequence shown in SEQ ID No.: 3 in the Sequence Listing.

[0065] (2) Preparation of lateral flow chromatogr...

Embodiment 2

[0074] Example 2. Specificity experiment of lateral flow chromatography sensor based on nucleic acid base mismatch

[0075] The lateral flow chromatography sensor based on nucleic acid base mismatch prepared in Example 1 was used to detect different metal ion solutions, and the specificity of the sensor was tested, wherein the concentration of Hg(II) was 1 μM. Other metal ions The concentration is 1 mM.

[0076] Drop the sample to be tested on the sample pad, and after about 5 minutes, the lateral flow chromatography sensor will display the detection result. Specific test results such as figure 2 As shown, the lateral flow chromatography sensor based on nucleic acid base mismatch only produces a signal response to mercury ions, indicating that the method has good specificity.

Embodiment 3

[0077] Embodiment 3, realize the quantitative detection of the lateral flow tomography sensor through the imaging analysis system of the smart phone

[0078]When the lateral flow chromatography sensor described in Example 1 according to the specific implementation manner of the present disclosure detects mercury ions, red lines will appear on the detection line and / or the quality control line. Therefore, by using the image containing the red line, by establishing a standard curve corresponding to the concentration of mercury ions in the mobile phone, the image containing the red line taken (or stored in the mobile phone in advance, or downloaded by the mobile phone) can be used to detect mercury. Quantitative detection of ion concentration.

[0079] (1) Establishment of standard curve

[0080] Prepare a series of mercury standard solutions with known concentrations diluted in multiples. The mercury ion concentrations in different mercury standard solutions are 0, 1nM, 10nM, 1...

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Abstract

The invention provides a mercury ion detection product, a mercury ion detection method and a smart phone imaging analysis system. A lateral flow chromatography sensor based on nucleic acid base mismatch, provided by the invention, comprises at least one nucleotide sequence shown in SEQ ID No.1-3 in a sequence table, and has high detection specificity and high sensitivity. According to the smart phone imaging analysis system matched with the lateral flow chromatography sensor, detection results displayed on the lateral flow chromatography sensor can be converted into concentration values of mercury ions by the mobile phone, and quantitative detection of the mercury ions in a sample is simply and efficiently realized. The mercury ion detection product, the mercury ion detection method and the smart phone imaging analysis system provided by the invention are very suitable for untrained personnel to carry out on-site tests, and provide great convenience for on-site tests of food safety andenvironmental safety.

Description

technical field [0001] The invention belongs to the technical field of biological detection, and in particular relates to a mercury ion detection product, method and smart phone imaging analysis system. Background technique [0002] Water-soluble divalent mercury ions are relatively common heavy metal risk factors in food safety and drinking water safety. They have strong bioaccumulation and are more harmful to the human body. System, digestive system, brain tissue and kidney tissue. Many countries and organizations have adjusted the maximum allowable upper limit of mercury ions in drinking water samples. For example, the World Health Organization (WHO) stipulates that the maximum allowable limit of mercury ions in drinking water should not exceed 6ng mL -1 (30nM), the US Environmental Protection Agency (Environmental Protection Agency, EPA) stipulates that the acceptable limit of mercury ions in drinking water is 2ng mL -1 (10nM), the European Union (EU) drinking water st...

Claims

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Application Information

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IPC IPC(8): G01N21/78G01N21/84
CPCG01N21/78G01N21/8483G01N2021/8494
Inventor罗云波许文涛程楠黄昆仑徐媛聪杨湛森
OwnerCHINA AGRI UNIV