Human pancreatic cancer neural invasion cell line

A technique for neural invasion and pancreatic cancer, applied in the field of cell lines, to achieve the effects of stable multiple passages, rich cell bank, and stable traits

Active Publication Date: 2018-11-23
郭世伟
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there is no report about the human pancreatic cancer nerve-infiltrating cell line of the present invention

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  • Human pancreatic cancer neural invasion cell line
  • Human pancreatic cancer neural invasion cell line
  • Human pancreatic cancer neural invasion cell line

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] The preparation of embodiment 1HPNI-1 cell line

[0033] Nude mice: 5, female, weighing 16.0±1.0 g, aged 5 weeks, raised in SPF environment.

[0034] A fresh resection specimen of clinical pancreatic cancer nerve invasion (male, 56 years old, pancreatic cancer nerve invasion) was obtained from Shanghai Changhai Hospital, and immediately immersed in pre-cooled sterile HBSS buffer (containing 500 U / ml penicillin G, 500 μg / ml sulfuric acid chain Mycin and 1.50 μg / ml Amphotericin B). In the biosafety cabinet, wash the specimen with fresh sterile HBSS buffer solution, cut into small pieces of 20-50 mg, and puncture and inoculate subcutaneously in the axillary back of nude mice. After inoculation, the animals were in good health and behaved normally. 40 days after inoculation, small tumor nodules were found under the skin of the inoculation site by touch, and the small nodules began to grow significantly 50 days after inoculation, and the tumor volume exceeded 300mm by 70 d...

Embodiment 2

[0037] The biological characteristic of embodiment 2HPNI-1 cell line

[0038] The invention adopts RPMI 1640 culture solution containing fetal bovine serum and insulin to cultivate HPNI-1 cells, so that the HPNI-1 cells can grow long-term in vitro and pass stably. When the cells are passed to more than 20 generations, the cell properties are gradually stabilized, and the relevant biology, genetics and tissue source identification are carried out until the 50th generation has the same stable properties. According to the experimental observation and verification, the cells grown in vitro are epithelial-like. Under the inverted microscope, the cells adhere to the wall and grow evenly, with different sizes and paving stone-like shapes. grow. Genetic studies have confirmed that the cells are aneuploid, with severe chromosome number and structural aberrations, consistent with the genetic characteristics of malignant tumors.

[0039] (1) Morphological observation

[0040] The cult...

Embodiment 3

[0047] Embodiment 3 HPNI-1 cell line is applied to establish animal model

[0048] The HPNI-1 cells were cultured and collected in vitro, and subcutaneously inoculated into BALB / C nude mice (5.0×10 6 cells, the cell suspension and Matrigel were mixed at a ratio of 1:1, and a total of 7 animals were inoculated), and the body weight and tumor size of the animals were investigated twice a week. About 5 days after inoculation, tumors began to form and grow. Tumor growth curves were drawn, where tumor volume = (length x width x width) ÷ 2. The experiment ended on the 39th day, when the tumor volume was 2013.9±263.5mm 3 , and the animals were euthanized with a tumor weight of 1.89±0.75 g. It can be seen that HPNI-1 cells can form tumors in nude mice and have tumorigenicity.

[0049] The excised specimen of Example 1 and the tumor formed by HPNI-1 cells subcutaneously inoculated in nude mice 20 days later were subjected to paraffin-embedded sections and HE staining. The results ...

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Abstract

The invention relates to a human pancreatic cancer neural invasion cell line which is preserved in the CCTCC (China Center for Type Culture Collection) and has a preservation number of CCTCC NO:C201622. The invention further provides application and a cell line establishment method of the cell line. The invention shows the advantages that the invention provides the human pancreatic cancer neural invasion cell line and a cell bank of the human pancreatic cancer is enriched. The human pancreatic cancer neural invasion cell line has tumorigenicity, and has strong tumorigenicity when being inoculated to a nude mouse; and tissue slices prompts that histological characteristics of the transplantation tumor are similar with that of a clinical specimen. The human pancreatic cancer neural invasioncell line can provide a material for further researching occurrence of the pancreatic cancer and a neural invasion mechanism, and can also provide a platform for screening a medicament for treating the pancreatic cancer and particularly inhibiting neural invasion of the pancreatic cancer. The human pancreatic cancer neural invasion cell line disclosed by the invention is stable in character and can be stably and repeatedly subjected to passage.

Description

technical field [0001] The invention relates to the technical field of cell lines, in particular to a human pancreatic cancer nerve-infiltrating cell line. Background technique [0002] Pancreatic cancer is one of the common malignant tumors of the digestive tract. The annual incidence rate of pancreatic cancer in my country is 5.1 / 100,000, which is a substantial increase compared with 20 years ago. The level of surgical treatment of pancreatic cancer in my country has improved significantly. The surgical resection rate of pancreatic cancer has risen from less than 15% to about 40%, and serious postoperative complications have decreased significantly. However, the 5-year survival rate of pancreatic cancer after surgery has not been significantly improved, and most reports are between 5% and 10%. Recurrence and liver metastases are common. These two factors are two difficult problems to be solved that restrict the effect of surgical treatment of pancreatic cancer. [0003]...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/09C12Q1/02A01K67/027C12R1/91
CPCA01K67/0271G01N33/5011C12N5/0693A01K2267/0331A01K2207/12A01K2227/105
Inventor 郭世伟
Owner 郭世伟
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